ZNF206 Antibody [Unconjugated]

ZNF206 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human ZNF206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with tubulin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ZNF206 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ZNF206
  Met1-Val283
  Accession # Q96SZ4
• Specificity: Detects human ZNF206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ZNF24 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZSCAN10
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ZNF206 Antibody [Unconjugated]

• Zfp206
• ZNF206
• ZSCAN10 zinc finger and SCAN domain containing 10
• ZSCAN10

Background

ZNF206 (zinc finger protein 206; also ZFP206) is an 80 kDa SCAN-zinc finger transcription factor that is active in embryonic stem cells and appears to positively regulate transcriptional regulators that maintain stem cell pluripotency. Human ZNF206 is 725 amino acids in length. It contains one SCAN box (aa 1‑71) that mediates oligomerization, followed by a proline-rich region (aa 104‑175) and 14 consecutive C2H2-type zinc fingers (aa 292‑719). Mouse ZNF206 apparently has multiple isoforms, none of which are well characterized. The human gene may also show alternate splicing. Human ZNF206 is 72% aa identical to mouse ZNF206.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZNF206 Antibody (AF4020)(2)

Publications using AF4020

• M Skamagki, C Correia, P Yeung, T Baslan, S Beck, C Zhang, CA Ross, L Dang, Z Liu, S Giunta, TP Chang, J Wang, A Ananthanar, M Bohndorf, B Bosbach, J Adjaye, H Funabiki, J Kim, S Lowe, JJ Collins, CW Lu, H Li, R Zhao, K Kim ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors Nat. Cell Biol., 2017-08-28;19(9):1037-1048. 2017-08-28 [PMID: 28846095]
• Yamane M, Fujii S, Ohtsuka S, Niwa H Zscan10 is dispensable for maintenance of pluripotency in mouse embryonic stem cells. Biochem Biophys Res Commun, 2015-11-22;468(4):826-31. 2015-11-22 [PMID: 26592664]

Bioinformatics

• Gene Symbol: ZSCAN10
• Uniprot:
  • Q96SZ4()