VIPR1/VPAC1 Antibody

Product Discontinued

VIPR1/VPAC1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EECDYVQMIEVQHKQCLEEAQLENETIGCSKMWDNLTCWPATPRGQVVVLACPLIFKLFSSIQGRNVSRSCTDEGWTHLEPGPYPIACGLDDKAASLDEQQTMFYGSVKTGY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VIPR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. ICC/IF reactivity reported in scientific literature (PMID: 27059672).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for VIPR1/VPAC1 Antibody

• FLJ41949
• HVR1
• II
• PACAP type II receptor
• PACAP-R2
• RDC1
• type II
• V1RG
• vasoactive intestinal peptide receptor 1
• vasoactive intestinal polypeptide receptor 1
• VIP R1
• VIP receptor, type I
• VIPR
• VIPR1
• VPAC1
• VPAC1R

Background

The Vasoactive Intestinal Polypeptide Receptor 1 (VPAC1) is a member of a family of three G-protein coupled receptors which mediate VIP (vasoactive intestinal peptide; a small neuropeptide) and PACAP (pituitary adenlyate cyclase activating peptide) function in a variety of tissues. Together, the VPAC1 and VPAC2 receptors compose a subtype of the VIP receptor. Expression of the VIP and PACAP receptors has been linked to the presence of human tumors. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate cyclase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VIPR1/VPAC1 Antibody (NBP1-89640)(1)

Publication using NBP1-89640

• Bertolini M, Pretzlaff M, Sulk M et al. VIP, whose receptor-mediated signalling may be defective in alopecia areata, provides protection from hair follicle immune privilege collapse. Br. J. Dermatol. 2016-04-05 [PMID: 27059672] (IHC-P, ICC/IF, Human)

Bioinformatics

• Gene Symbol: VIPR1