| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF643-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47491SS | |||
| Azide and BSA Free | NBP2-81005 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF809-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-28406 | |||
| FITC | NBP1-28405 | |||
| PE | NBP1-28407 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB809-SP | |||
| Carrier Free | MAB809-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6434-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74840 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8091-SP | |||
| Carrier Free | FAB8091V-100UG | |||
| Carrier Free | FAB8091U-100UG | |||
| Carrier Free | FAB8091T-100UG | |||
| Carrier Free | FAB8091S-100UG | |||
| Carrier Free | FAB8091R-100UG | |||
| Carrier Free | FAB8091N-100UG | |||
| Carrier Free | FAB8091G-100UG | |||
![Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Western-Blot-NBP1-47491-img0006.jpg "Western Blot: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate.")
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-Paraffin-NBP1-47491-img0004.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Human placenta tissues using VCAM1 mouse mAb.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-Paraffin-NBP1-47491-img0005.jpg)
![Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Breast carcinoma, cytoplasmic staining. IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-NBP1-47491-img0007.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - FFPE section of human lung cancer. Image at 20X magnification. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Immunohistochemistry-Paraffin-NBP1-47491-img0009.jpg)
![Simple Western: VCAM-1/CD106 Antibody (6G9) [NBP1-47491] - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/mL of HUVEC lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-6G9-Simple-Western-NBP1-47491-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Enhanced expression of endothelial SENP1 & GATA2 correlates w/ graft arteriosclerosis (GA) progression.Similarly sized human coronary arteries w/ GA from chronically rejecting heart allografts or w/out disease from non-transplanted hearts collected & evaluated by histological analysis. (a,b) Dramatically increased expression of endothelial SENP1 detected in the diseased vessel wall. Endothelial SENP1 expression is demonstrated by immunofluorescence analysis of coronary artery cross-sections that stained for SENP1 & the endothelial marker PECAM-1 w/ DAPI labelling of the nuclei. Representative images are shown in (a) w/ quantification data in (b). Bar represents 50 μm. (c) Induction of endothelial adhesion molecules resulted in a similar augmented pattern as endothelial SENP1. Representative images of immunofluorescence staining for VCAM-1 or P-selectin & PECAM-1 in coronary arteries w/ DAPI counterstaining are shown. Bar represents 50 μm. (d,e) Expression of endothelial GATA2 elevated w/ the progression of aggravated rejection. Representative images of the immunofluorescence staining of coronary arteries for GATA2 & PECAM-1 together w/ DAPI nuclear staining are shown in (d) w/ quantification data in (e). Negative SENP1 & GATA2 staining in the isotype or blocking peptide controls are also shown in (a) & (d). Bar represents 50 μm. Data presented in (b,e) are the mean±s.e.m. from five separate clinical specimens per group as indicated. *P<0.05, **P<0.01 & ***P<0.0001; one-way ANOVA followed by Bonferroni test. GA, graft arteriosclerosis; PECAM, platelet/endothelial cell adhesion molecule; SENP, sentrin-specific protease; VCAM, vascular cell adhesion molecule. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47491_mouse-monoclonal-vcam-1-cd106-antibody-6g9-310202416161834.jpg)
![Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (6G9) - BSA Free [NBP1-47491] - Loss of endothelial SENP1 inhibits EC activation.(a) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin & PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 μm. (b–e) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. Flow cytometry analysis of ICAM-1, VCAM-1 & P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1 beta treatment. Representative histograms are shown in (b) with the quantification of mean intensity in (c–e). (f–h) Overexpression of the catalytically inactive form of SENP1 (SENP1-Mut) inhibits the induction of adhesion molecules in HUVECs. HUVECs were infected by Ad-SENP1-Mut or vector control (Ad-LacZ) for 24 h, treated with pro-inflammatory cytokines & analysed by flow cytometry in the same way as MAECs. Representative histograms of ICAM-1 & VCAM-1 are shown in (f) with the quantification of mean intensity in (g,h). Data are presented as the mean±s.e.m. from at least three independent experiments. *P<0.05 & **P<0.01; two-way ANOVA followed by Bonferroni post-test. MAEC, mouse aortic endothelial cell. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms15426), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-47491_mouse-monoclonal-vcam-1-cd106-antibody-6g9-31020241616185.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Veins and venules in the interalveolar stroma of porcine mammary gland are positive for the human VCAM-1. Antigen retrieval by Proteinase K. AlexaFluor 488 anti-mouse antibody was used as a secondary detection antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-G11B1-Immunohistochemistry-Paraffin-NBP1-28404-img0005.jpg)
![Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - TNF alpha stimulated human endothelial cell line HUV-EC-C was stained with Mouse Anti-Human CD106-UNLB followed by Goat Anti-Mouse IgG1, Human ads-FITC.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-G11B1-Flow-Cytometry-NBP1-28404-img0006.jpg)
![Flow Cytometry: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Analysis using the FITC conjugate of NBP1-28404. Staining of TNF alpha stimulated endothelial cell line HUV-EC-C.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-1-G11B1-Flow-Cytometry-NBP1-28404-img0003.jpg)
![Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Knockdown of RPN2 decreased luminal B BC cell dissemination in vivo. Luminal B ER+ T47D cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting RPN2 (siRNA-RPN2) were injected + estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos with green fluorescent blood vessels & analyzed as described in materials & methods. (A) Migration in vitro (n = 6). (B)In vivo dissemination of transfected T47D in presence of E2 ± Neu (n = 23–26). Scale bar = 100 µm. (n = 23–26). (C) Western blot analysis for confirmation of siRNA-RPN2 transfection & VCAM-1, ICAM-1, & MUC-1 expression. (D) Focal adhesions area (n = 5–6). Scale bar = 10 µm. (E) Proliferation (n = 6). Representative images of zebrafish embryos with disseminated luminal B T47D BC cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated T47D & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, ***P < 0.001, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28404_mouse-monoclonal-vcam-1-cd106-antibody-1-g11b1-310202416154617.jpg)
![Western Blot: VCAM-1/CD106 Antibody (1.G11B1) [NBP1-28404] - Knockdown of U2AF1 decreased luminal A BC cell dissemination in vivo. Luminal A ER+ MCF-7 cells, transfected with negative control siRNA (siRNA-C) or siRNA targeting U2AF1 (siRNA-U2AF1) were injected in presence of estradiol (E2) ± neutrophils (Neu) into zebrafish transgenic embryos, with green fluorescent blood vessels, & analyzed as described in materials & methods. (A) Migration in vitro (n = 6–12). (B)In vivo dissemination in presence of E2 ± Neu (n = 38–41). Scale bar = 100 µm. (C) Western blot analysis for confirmation of siRNA-U2AF1 transfection & ICAM-1, VCAM-1, & MUC-1 expression. (D) Focal adhesion area (n = 7). Scale bar = 10 µm. (E) Proliferation in vitro (n = 12). Representative images of zebrafish embryos with disseminated MCF-7 cells & immunocytochemistry analysis of vinculin expression are shown. Arrows show disseminated MCF-7 cells & arrowheads show focal adhesions. BV = blood vessels. Data are presented as mean ± SEM. Two-tailed Student’s t-test *P < 0.05, **P < 0.01, ns, not significant. Data are represented of at least two independent experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33330095), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-28404_mouse-monoclonal-vcam-1-cd106-antibody-1-g11b1-31020241617356.jpg)
![Western Blot: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of VCAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: human spleen tissue lysate](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-SA05-04-Western-Blot-NBP2-67292-img0005.jpg "Western Blot: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of VCAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: human spleen tissue lysate")
![Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Staining VCAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-SA05-04-Immunocytochemistry-Immunofluorescence-NBP2-67292-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-VCAM-1/CD106 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-SA05-04-Immunohistochemistry-Paraffin-NBP2-67292-img0007.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded human spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-SA05-04-Immunohistochemistry-Paraffin-NBP2-67292-img0002.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded human tonsil tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-SA05-04-Immunohistochemistry-Paraffin-NBP2-67292-img0003.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of paraffin-embedded mouse spleen tissue using anti-VCAM1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-SA05-04-Immunohistochemistry-Paraffin-NBP2-67292-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VCAM-1/CD106 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-SA05-04-Immunohistochemistry-Paraffin-NBP2-67292-img0006.jpg)
![Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Western-Blot-NBP2-03600-img0021.jpg "Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VCAM1.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunocytochemistry-Immunofluorescence-NBP2-03600-img0015.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0019.jpg)
![Western Blot: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Western-Blot-NBP2-03600-img0020.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0016.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - CD106 / VCAM1 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0017.jpg)
![Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (3H10) [NBP2-03600] - Staining of paraffin-embedded Human Kidney tissue using anti-VCAM1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/VCAM-1-CD106-Antibody-3H10-Immunohistochemistry-Paraffin-NBP2-03600-img0018.jpg)
