USP28 Antibody

Western Blot: USP28 Antibody [NBP2-76973] - Western blot analysis of USP28 on different cell lysates using anti-USP28 antibody at 1/500 dilution.Positive control:Lane1: K562Lane2: A549Lane3: Daudi

Flow Cytometry: USP28 Antibody [NBP2-76973] - Flow cytometric analysis of MG-63 cells with USP28 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Discontinued

USP28 Antibody Summary

• Immunogen: Recombinant protein within human USP28 aa 550-700. (SwissProt: Q96RU2 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP28
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Western Blot 1:200-1:1000
• Theoretical MW: 122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP28 Antibody

• Deubiquitinating enzyme 28
• EC 3.1.2.15
• EC 3.4.19.12
• KIAA1515ubiquitin carboxyl-terminal hydrolase 28
• ubiquitin specific peptidase 28
• ubiquitin specific protease 28
• ubiquitin thioesterase 28
• Ubiquitin thiolesterase 28
• Ubiquitin-specific-processing protease 28
• USP28

Background

The ubiquitin-dependent protein degradation pathway is essential for proteolysis of intracellular proteins and peptides. Enzymes that remove ubiquitin from ubiquitin-conjugated peptides, like USP28, affect the fate and degradation of intracellular proteins and are essential for maintenance of cell-free ubiquitin pools (Valero et al., 2001).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP28