Tryptase epsilon/BSSP-4/PRSS22 Antibody (246744)

• Reactivity: Mu
• Applications: WB, IP
• Clone: 246744
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Tryptase epsilon/BSSP-4/PRSS22 Antibody (246744) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Tryptase epsilon /BSSP‑4
  Ala33-Ser306 (predicted)
  Accession # Q7TML0
• Specificity: Detects mouse Tryptase epsilon /BSSP‑4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PRSS22
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptase epsilon/BSSP-4/PRSS22 Antibody (246744)

• BSP-2
• BSSP4
• BSSP-4
• hBSSP-4
• MGC9599
• prosemin
• protease, serine S1 family member 22
• protease, serine, 22
• PRSS22
• serine protease 22
• serine protease 26
• SP001LA
• Tryptase epsilon
• UNQ302/PRO343

Background

Tryptase epsilon , brain specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2) are different names given for the same serine protease that is encoded by the PRSS22 gene (1‑3). Initially identified having brain-specific expression, mouse Tryptase epsilon is preferentially expressed in epithelium-rich tissues such as the lung and eye, which is similar to its human counterpart (3). The mouse protein is synthesized with a signal peptide (amino acid residues 1 to 32), a pro peptide (residues 33 to 49) and a mature chain (residues 50 to 306) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing result indicates that the purified protein corresponds to the pro enzyme. After activation with thermolysin, the enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The thioester activity is inhibited by 2 mM AEBSF (R&D Systems, Catalog # EI001), a general serine protease inhibitor, and by recombinant human Serpin A5 (R&D Systems, Catalog # 1266-PI).

1. Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
2. Davies, B.J. et al. (1998) J. Biol. Chem. 273:23004.
3. Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptase epsilon/BSSP-4/PRSS22 Antibody (MAB2059)(1)

Publication using MAB2059

• Sa S, Wong L, McCloskey KE. Combinatorial fibronectin and laminin signaling promote highly efficient cardiac differentiation of human embryonic stem cells. Biores Open Access 8/1/2014 [PMID: 25126479]

Bioinformatics

• Gene Symbol: PRSS22
• Uniprot:
  • Q7TML0()