Human Troponin I Type 3 (cardiac) ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard curve. Troponin I (Human) ELISA Kit is an enzyme immunoassay for the quantitative measurement of troponin I. |
| Standard Curve Range |
0 to 75 ng/mL |
| Sensitivity |
1 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 5.04% |
| Intra-Assay |
CV% < 3.78% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TNNI3 |
Applications/Dilutions
| Dilutions |
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| Application Notes |
Useful for quantification. |
| Publications |
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Packaging, Storage & Formulations
Kit Components
|
Components
|
- Antibody-Coated Wells: Microtiter wells coated with mouse monoclonal anti-TnI.
- Component
- cTnI Enzyme Conjugate Reagent: Contains mouse monoclonal anti-TnI conjugatedto horseradish peroxidase in Tris Buffer-BSA solution with preservatives
- Reference Standard Set: Contains 0, 2.0, 7.5, 30, and 75 ng/ml TnI, lyophilized.
- Stop Solution: Contains diluted hydrochloric acid (1N HCl)
- TMB Reagent: Contains one-step TMB solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Troponin I Type 3 (cardiac) ELISA Kit (Colorimetric)
Background
Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for Troponin I Type 3 (cardiac) ELISA Kit (KA0233)(6)
Showing Publications 1 -
6 of 6.
| Publications using KA0233 |
Applications |
Species |
| Zhao L, Zhang B. Doxorubicin induces cardiotoxicity through upregulation of death receptors mediated apoptosis in cardiomyocytes. Sci Rep 2017-03-16 [PMID: 28300219] |
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| Wang S, He W, Wang C. MiR-23a regulate the vasculogenesis of coronary artery disease by targeting EGFR. Cardiovasc Ther 2016-04-17 [PMID: 27085964] |
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| Lagerqvist EL, Finnin BA, Elliott DA et al. Comparing mouse and human pluripotent stem cell derived cardiac cells: Both systems have advantages for pharmacological and toxicological screening. J Pharmacol Toxicol Methods. 2015-05-06 [PMID: 25957031] |
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| Doherty KR, Talbert DR, Trusk PB et al. Structural and functional screening in human induced-pluripotent stem cell-derived cardiomyocytes accurately identifies cardiotoxicity of multiple drug types. Toxicol Appl Pharmacol. 2015-04-02 [PMID: 25841593] |
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| Wang F, Long G, Zhao C et al. Plasma microRNA-133a is a new marker for both acute myocardial infarction and underlying coronary artery stenosis. J Transl Med. 2013-09-23 [PMID: 24053180] |
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| Long G, Wang F, Duan Q et al. Circulating miR-30a, miR-195 and let-7b Associated with Acute Myocardial Infarction PLoS One. 2012-12-07 [PMID: 23236408] |
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Product General Protocols
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FAQs for Troponin I Type 3 (cardiac) ELISA Kit (KA0233). (Showing 1 - 2 of 2 FAQs).
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Can I use human Troponin ELISA kit for measuring rat troponin?
- The amino acids sequence of Human Troponin 1 Type 3 shares 93% similarity with the sequence of rat, therefore, so this kit may be expected to detect rat Troponin 1 Type 3.
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.