Troponin I Type 3 (cardiac) Antibody

Western Blot: Troponin I Type 3 (cardiac) Antibody [NBP2-29658] - 20 ug of mouse heart lysate showing specific immunolabeling of the ~25k cardiac troponin I protein.

Product Discontinued

Troponin I Type 3 (cardiac) Antibody Summary

• Immunogen: Fusion protein of complete mouse cardiac troponin 1. Accession # P48787.
• Specificity: Specific for the ~25k cardiac troponin I protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNNI3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Application Notes: Suggested dilution is 1:2,000 although higher dilutions can sometimes be used as cardiac troponin I expression is quite high in cardiac muscle.
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Neat whole antisera
• Preservative: No Preservative
• Purity: Unpurified

Alternate Names for Troponin I Type 3 (cardiac) Antibody

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Biological Significance: Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the calcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by extension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosphorylation of TnI (largely at Ser43) inhibits the actin-cross bridge reaction and reduces the Ca++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in the phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNI3
• Entrez:
  • 21954(Mouse)
• Uniprot:
  • P48787(Mouse)