Troponin I Type 3 (cardiac) Antibody (228)

Product Discontinued

Troponin I Type 3 (cardiac) Antibody (228) Summary

• Immunogen: free human cTnI, native human cTn complex, or human cTnI peptides.
• Specificity: This is reactive against troponin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNNI3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Antibody combinations?for quantitative sandwich immunoassay: NB100-73084 + NB100-73087 (mixture for coating) - NB110-2546 + NB100-73086 (mixture for detection)

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Troponin I Type 3 (cardiac) Antibody (228)

• Cardiac troponin I
• CMD1FF
• CMD2A
• CMH7MGC116817
• CTnI
• familial hypertrophic cardiomyopathy 7
• RCM1
• TNNC1
• TNNC1troponin I, cardiac
• TNNI3,
• troponin I type 3 (cardiac)
• troponin I, cardiac muscle
• Troponin I/TNNI3

Background

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNNI3
• Entrez:
  • 7137(Human)