TMPRSS4 Antibody

Immunohistochemistry-Paraffin: TMPRSS4 Antibody [NBP1-50175] - Staining of paraffin-embedded human colon with TMPRSS4 antibody at concentration 15 ug/ml.

Immunohistochemistry-Paraffin: TMPRSS4 Antibody [NBP1-50175] - Staining of paraffin-embedded human colon with TMPRSS4 antibody at concentration 15 ug/ml.

Product Discontinued

TMPRSS4 Antibody Summary

• Immunogen: TMPRSS4 Antibody is made to a synthetic 16 aa peptide from internal region of human TMPRSS4.
• Specificity: Human TMPRSS4. BLAST analysis of the peptide immunogen for TMPRSS4 Antibody showed no homology with other human proteins, except CDH23 (56%).
• Predicted Species: Mouse (94%), Orangutan (100%), Guinea Pig (94%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMPRSS4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 15-20 ug/ml

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Marmoset (100%), Rat (88%), Bovine (88%), Equine (88%), Rabbit (88%), Porcine (88%), Opossum (88%), Galago (81%), Hamster (81%), Canine (81%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TMPRSS4 Antibody

• CAPH2
• Channel-activating protease 2
• EC 3.4.21
• EC 3.4.21.4
• EC 3.4.21.43
• Membrane-type serine protease 2
• MT-SP2
• TMPRSS3EC 3.4.21.-
• TMPRSS4
• transmembrane protease serine 4
• transmembrane protease, serine 4
• transmembrane serine protease 3
• Type II membrane serine protease

Background

TMPRSS4, previously known as TMPRSS3, is a 437 amino acid type II transmembrane serine protease located on human chromosome 11q23.3 that encodes 5 isoforms containing a complete coding sequence. This serine protease has a predicted molecular weight of 48 kDa with 2 glycosylation sites and the cleaved soluble protease domain has been detected in cell culture media. Functions of TMPRSS4 include embryo development, viral infection, and cancer. TMPRSS4 plays a role in the epithelial-mesenchymal transition (EMT) process and mediates cell invasion, migration, proliferation and metastasis. Overexpression of TMPRSS4 has been reported for multiple solid tumors including pancreatic, ovarian, thyroid, colorectal, lung, breast, cervical, gallbladder, gastric, and liver cancer, and has been associated with poor overall survival and reduced time to tumor progression (1,2).
TMPRSS4 has also been shown to play a role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 or potentially by TMPRSS4 at the cell surface to facilitate viral entry (3).
References

1. L de Aberasturi A, Calvo A. (2015) TMPRSS4: An Emerging Potential Therapeutic Target in Cancer. Br J Cancer. 112(1):4-8. PMID: 25203520

2. Zeng P., Zhang P., Zhou L., Tang M., Shen Y., Jin J., Zhu Y., Chen M. (2016) TMPRSS4 as an emerging potential poor prognostic factor for solid tumors: A systematic review and meta-analysis. Oncotarget. 7:76327-76336. PMID: 27344186

3. Zang F, Castro MFG, McCune BT, Zeng Q, Rothlauf PW, Sonnek NM, Liu Z, Brulois KF, Wang X, Greenberg HB, Diamond MS, Ciorba MA, Whelan SPJ, and Ding S. (2020) TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes. Sci Immunol. 5(47): eabc3582. PMID: 32404436

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMPRSS4
• Entrez:
  • 56649(Human)
• Uniprot:
  • Q9NRS4(Human)