TCR gamma/delta Antibody (GL-3) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
GL-3
Clonality
Monoclonal
Host
Hamster
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

TCR gamma/delta Antibody (GL-3) [Alexa Fluor® 594] Summary

Immunogen
Mouse intraepithelial lymphocytes
Specificity
Detects mouse TCR gamma /δ.
Isotype
IgG2 Kappa
Clonality
Monoclonal
Host
Hamster
Gene
Cd3d
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCR gamma/delta Antibody (GL-3) [Alexa Fluor® 594]

  • CD3d
  • delta polypeptide
  • T cell receptor gamma locus
  • T3D
  • T-cell antigen receptor, gamma polypeptide
  • T-cell rearranging gene, gamma
  • T-cell receptor, gamma cluster
  • TCR gamma/delta
  • TCRG
  • TiT3
  • TRG

Background

The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).
  1. Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Isotype Controls

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Bioinformatics

Gene Symbol Cd3d