TCR gamma/delta Antibody (GL-3/5E11) [Allophycocyanin]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Hamster Anti-Mouse TCR ?/d APC-conjugated Monoclonal Antibody (Catalog # FAB7297A) or (B) Hamster IgG Allophycocyanin Control. View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

TCR gamma/delta Antibody (GL-3/5E11) [Allophycocyanin] Summary

• Immunogen: Mouse intraepithelial lymphocytes
• Specificity: Detects mouse TCR gamma /δ.
• Source: N/A
• Isotype: IgG2 Kappa
• Clonality: Monoclonal
• Host: Hamster
• Gene: Cd3d
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCR gamma/delta Antibody (GL-3/5E11) [Allophycocyanin]

• delta polypeptide
• T cell receptor gamma locus
• T3D
• T-cell antigen receptor, gamma polypeptide
• T-cell rearranging gene, gamma
• T-cell receptor, gamma cluster
• TCR gamma/delta
• TCRG
• TiT3
• TRG

Background

The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).

1. Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Cd3d