Synaptotagmin 6 Antibody (S270-47)

Western Blot: Synaptotagmin VI Antibody (S270-47) [NBP2-22421] - analysis of Rat brain lysates showing detection of Synaptotagmin 6 protein using Mouse Anti-Synaptotagmin 6 Monoclonal Antibody, Clone S270-47 . Primary Antibody: Mouse Anti-Synaptotagmin 6 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.

Immunocytochemistry/Immunofluorescence: Synaptotagmin VI Antibody (S270-47) [NBP2-22421] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-6 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X.

Product Discontinued

Synaptotagmin 6 Antibody (S270-47) Summary

• Immunogen: Fusion protein amino acids 246-333 (Cytoplasmic C2A domain) of mouse Synaptotagmin-6
• Localization: Cytoplasmic Vesicle
• Specificity: Detects approx 55kDa. Does not cross-react with other synaptotagmins.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYT6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Synaptotagmin-6 Antibody was sufficient for detection of Synaptotagmin-6 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Synaptotagmin 6 Antibody (S270-47)

• synaptotagmin-6
• sytVI

Background

Synaptotagmins, such as SYT6, share a common domain structure that includes a transmembrane domain and a cytoplasmicregion composed of 2 C2 domains. Some synaptotagmins are involved in synaptic membrane fusion, while others have amore general function in endocytosis. For further information on synaptotagmins, see MIM 185605.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYT6
• Uniprot:
  • Q9R0N8(Mouse)