Synaptotagmin 5 Antibody

Western Blot: Synaptotagmin 5 Antibody [NBP2-26145] - NBP2-26145 (1ug/ml) staining of Olfactory bulb lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Synaptotagmin 5 Antibody Summary

• Description: Novus Biologicals Goat Synaptotagmin 5 Antibody (NBP2-26145) is a polyclonal antibody validated for use in WB and ELISA. Anti-Synaptotagmin 5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SYIDKVQPEIEELD corresponding to internal region according to NP_058604.1.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SYT5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in human brain (Olfactory bulb) lysates (calculated MW of 43.1 kDa band according to NP_058604.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Synaptotagmin 5 Antibody

• synaptotagmin Vsynaptotagmin 5
• synaptotagmin-5
• sytV

Background

May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synaptotagmin 5 Antibody (NBP2-26145)(1)

Publication using NBP2-26145

• Hudson AW, Birnbaum MJ. Identification of a nonneuronal isoform of synaptotagmin. Proc Natl Acad Sci U S A 1995-06-20 [PMID: 7597049]

Bioinformatics

• Gene Symbol: SYT5
• Entrez:
  • 6861(Human)
  • 53420(Mouse)
  • 54309(Rat)