![Western Blot: Synaptotagmin 3 Antibody (S278-19) [NBP2-22395] - analysis of Rat Brain Membrane showing detection of 75 kDa Synaptotagmin-3 protein using Mouse Anti-Synaptotagmin-3 Monoclonal Antibody, Clone S278-19 . Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptotagmin-3 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 75 kDa.](http://images.novusbio.com/fullsize/Synaptotagmin-3-Antibody-S278-19-Western-Blot-NBP2-22395-img0001.jpg "Western Blot: Synaptotagmin 3 Antibody (S278-19) [NBP2-22395] - analysis of Rat Brain Membrane showing detection of 75 kDa Synaptotagmin-3 protein using Mouse Anti-Synaptotagmin-3 Monoclonal Antibody, Clone S278-19 . Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Synaptotagmin-3 Monoclonal Antibody at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 75 kDa.")
| Immunogen | Fusion protein amino acids 442-533 (Cytoplasmic C2B domain) of mouse Synaptotagmin-3 |
| Localization | Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane |
| Specificity | Detects approx 75kDa. Does not cross-react with other Synaptotagmins. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SYT3 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/ml of Synaptotagmin-3 Antibody was sufficient for detection of Synaptotagmin-3 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Synaptotagmin 3 Antibody (S278-19) [NBP2-22395] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/Synaptotagmin-3-Antibody-S278-19-Immunocytochemistry-Immunofluorescence-NBP2-22395-img0002.jpg "Immunocytochemistry/Immunofluorescence: Synaptotagmin 3 Antibody (S278-19) [NBP2-22395] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Nucleus. Magnification: 60X.")
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