SURF2 Overexpression Lysate

Images

 
Western Blot: SURF2 Overexpression Lysate (Adult Normal) [NBL1-16621] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SURF2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SURF2 Overexpression Lysate Summary

Description
Transient overexpression lysate of surfeit 2 (SURF2) The lysate was created in HEK293T cells, using Plasmid ID RC204095 and based on accession number NM_017503. The protein contains a C-MYC/DDK Tag.
Gene
SURF2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
29.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SURF2 Overexpression Lysate

  • SURF-2
  • surfeit 2
  • surfeit locus protein 2

Background

This gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity. The gene shares a bidirectional promoter with SURF1, which is located on the opposite strand. The function of the encoded protein from this housekeeping gene has not yet been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-94912
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-87818
Species: Hu
Applications: WB, IHC, IHC-P
NB100-2277
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP1-59452
Species: Hu
Applications: WB
NBP2-20932
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-57162
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-38290
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP2-30949
Species: Hu
Applications: IHC, IHC-P
NBP2-16366
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84999
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-81293
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB500-525
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-31113
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90824
Species: Hu
Applications: WB, ICC/IF
NBP2-70694
Species: Hu
Applications: WB, ICC/IF, IHC
NBP1-97512
Species: Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-92677
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-69571
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for SURF2 Lysate (NBL1-16621) (0)

There are no publications for SURF2 Lysate (NBL1-16621).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SURF2 Lysate (NBL1-16621) (0)

There are no reviews for SURF2 Lysate (NBL1-16621). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SURF2 Lysate (NBL1-16621) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-16621

Additional SURF2 Products

Bioinformatics Tool for SURF2 Lysate (NBL1-16621)

Discover related pathways, diseases and genes to SURF2 Lysate (NBL1-16621). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Pathways for SURF2 Lysate (NBL1-16621)

View related products by pathway.

PTMs for SURF2 Lysate (NBL1-16621)

Learn more about PTMs related to SURF2 Lysate (NBL1-16621).

Blogs on SURF2

There are no specific blogs for SURF2, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SURF2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SURF2