STOML3 Antibody

Western Blot: STOML3 Antibody [20760002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight [kDa]: 32 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

Immunohistochemistry: STOML3 Antibody [20760002] - Cells in lymphoid follicles were negative. Endothelial cells were distinctly stained. Strong membranous positivity was observed in fallopian tube, hepatocytes and gallbladder. Among malignancies, most tissues displayed moderate cytoplasmic staining. Distinct additional membranous positivity was observed in several adenocarcinomas of colon, breast, cervix, liver, ovary and also in melanomas. Image and statement courtesy of the Human Protein Atlas (HPA).

Western Blot: STOML3 Antibody [20760002] - Western Blot was performed using affinity purified SEQer STOML3 antibody, aa(192-291) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer STOML3 antibody, aa(192-291) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

ELISA: STOML3 Antibody [20760002]

Product Discontinued

STOML3 Antibody Summary

• Immunogen: This antibody is specific for the C Terminus Region of the target protein.
• Specificity: This product is specific for Human STOML3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STOML3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:5000-1:20000
• Application Notes: This antibody is useful in ELISA, Immunohistochemistry-Paraffin and Western Blot.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs.

Alternate Names for STOML3 Antibody

• Epb7.2l
• erythrocyte band 7 integral membrane protein
• SLP-3
• SROprotein 7.2B
• stomatin (EPB72)-like 3
• stomatin-like protein 3
• STOML3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STOML3 Antibody (20760002)(2)

Publications using 20760002

• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation, and Pathway Interconnections With Obesity J Proteome Res. 2017-01-06 [PMID: 27769113] (MiAr)
  
  Details:
  Analysis is performed on plasma proteomic data to identify how obesity can alter pathways and to highlight the risk factor for disease in subjects with a high body mass index.
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays J Proteome Res. 2013-05-03 [PMID: 23541305] (MiAr)
  
  Details:
  A novel method using antibody microarrays is used to detect autoantibody-antigen complexes that can potentially be useful for detection and characterization of diseases.

Bioinformatics

• Gene Symbol: STOML3
• Uniprot:
  • NM_145286(Human)