Soggy-1/DkkL1 Antibody [Unconjugated]

Western blot shows lysate of mouse testis tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Soggy-1/DkkL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Soggy-1/DkkL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Soggy-1/DkkL1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Soggy-1/DkkL1
  Leu21-Leu230
  Accession # AAF02679
• Specificity: Detects mouse Soggy-1/DkkL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human Soggy-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DKKL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Soggy-1/DkkL1 Antibody [Unconjugated]

• Cancer/testis antigen 34
• CT34dickkopf-like 1 (soggy)
• dickkopf-like 1
• DkkL1
• Protein soggy-1
• SGY
• SGY1
• SGY-1dickkopf-like protein 1
• SGY1soggy
• Soggy1
• Soggy-1

Background

Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The mouse Soggy-1 cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence (2). Mouse Soggy-1 shares 65% and 90% aa sequence identity with human and rat Soggy-1, respectively, and approximately 15% aa sequence identity with mouse Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3‑5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).

1. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
2. Krupnik, V.E. et al. (1999) Gene 238:301.
3. Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
4. Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
5. Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
6. Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Soggy-1/DkkL1 Antibody (AF1508)(2)

Publications using AF1508

• Zhuang XJ, Hou XJ, Liao SY SLXL1, a Novel Acrosomal Protein, Interacts with DKKL1 and Is Involved in Fertilization in Mice. PLoS ONE, 2011-06-15;6(6):e20866. 2011-06-15 [PMID: 21698294] (IHC-Fr, Western Blot, Mouse)
• Kohn MJ, Kaneko KJ, DePamphilis ML DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis. Mol. Reprod. Dev., 2005-08-01;71(4):516-22. 2005-08-01 [PMID: 15892050] (IHC-P, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: DKKL1
• Uniprot:
  • AAF02679()