SGK2 Overexpression Lysate (Native)

Images

 
Western Blot: SGK2 Overexpression Lysate (Adult Normal) [NBL1-15907] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SGK2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SGK2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204101 and based on accession number NM_170693. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens serum/glucocorticoid regulated kinase 2 (SGK2), transcript variant 1, mRNA.
Gene
SGK2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SGK2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SGK2 Overexpression Lysate (Native)

  • dJ138B7.2
  • EC 2.7.11
  • EC 2.7.11.1
  • H-SGK2
  • serine/threonine-protein kinase Sgk2
  • serum/glucocorticoid regulated kinase 2
  • Serum/glucocorticoid-regulated kinase 2
  • SGK2
  • Sgkl

Background

This gene encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Two alternate transcripts encoding two different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (1) contains an alternate in-frame exon in the 5' coding region, compared to variant 2. Isoform alpha has a shorter N-terminus, as compared to isoform beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-19010
Species: Hu
Applications: WB, IHC, IHC-P
NLS7240
Species: Hu, Mu, Rt, Bt, Ca, Ha
Applications: IHC-P
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-54974
Species: Hu
Applications: WB
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB110-81529
Species: Hu, Mu, Rt, Op, Pm, Rb
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IP, IF
MAB864
Species: Hu, Mu, Rt
Applications: WB, ICC
NB100-2383
Species: Hu
Applications: WB, ICC/IF
NB100-2450
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-12897
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00003753-M01
Species: Hu
Applications: WB, ELISA, RNAi
H00003738-M01
Species: Hu
Applications: WB, ELISA
NBP2-48740
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-84938
Species: Hu
Applications: IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB100-93520
Species: Hu
Applications: WB, ICC/IF, PEP-ELISA
NBP1-62518
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-60082
Species: Hu
Applications: WB, IHC, IHC-P

Publications for SGK2 Lysate (NBL1-15907) (0)

There are no publications for SGK2 Lysate (NBL1-15907).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SGK2 Lysate (NBL1-15907) (0)

There are no reviews for SGK2 Lysate (NBL1-15907). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SGK2 Lysate (NBL1-15907) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SGK2 Products

Bioinformatics Tool for SGK2 Lysate (NBL1-15907)

Discover related pathways, diseases and genes to SGK2 Lysate (NBL1-15907). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SGK2 Lysate (NBL1-15907)

Discover more about diseases related to SGK2 Lysate (NBL1-15907).
 

Pathways for SGK2 Lysate (NBL1-15907)

View related products by pathway.

PTMs for SGK2 Lysate (NBL1-15907)

Learn more about PTMs related to SGK2 Lysate (NBL1-15907).
 

Research Areas for SGK2 Lysate (NBL1-15907)

Find related products by research area.

Blogs on SGK2

There are no specific blogs for SGK2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

SGK2 Antibody
NBP1-86102

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SGK2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SGK2
Entrez