SERTAD3 Overexpression Lysate (Native)

Western Blot: SERTAD3 Overexpression Lysate (Adult Normal) [NBL1-15861] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SERTAD3.

• Applications: WB

SERTAD3 Overexpression Lysate (Native) Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC204122 and based on accession number NM_013368. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens SERTA domain containing 3 (SERTAD3), transcript variant 1, mRNA.
• Gene: SERTAD3

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
• Theoretical MW: 21.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for SERTAD3

• Type: Overexpression
• Protein State: Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SERTAD3 Overexpression Lysate (Native)

• RBT1Replication protein-binding trans-activator
• RPA-binding trans-activator
• SERTA domain containing 3
• SERTA domain-containing protein 3

Background

The protein encoded by this gene was identified in a yeast two-hybrid assay employing the second subunit of human replication protein A as bait. It is localized to the nucleus and its expression is significantly higher in cancer cell lines compared to normal cell lines. This protein has also been shown to be a strong transcriptional co-activator. Alternative splicing has been observed at this locus and two variants, both encoding the same protein, have been identified. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SERTAD3