SAH3 Antibody

Images

 
Western Blot: SAH3 Antibody [NB100-401] - Samples: NETN whole cell lysate (20 ug for WB; 1 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-SAH3 antibody used at the indicated concentration for WB ...read more
Immunocytochemistry/ Immunofluorescence: SAH3 Antibody [NB100-401] - SAH3 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SAH3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human adenosylhomocysteinase 3 (KIAA0828) using the numbering given in entry NP_056143.1 (GeneID 23382).
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AHCYL2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunoprecipitation 1-4 ug/mg of lysate
Application Notes
This SAH3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, and Western Blot, where a band should be seen at ~70-80 kDa. In ICC/IF, cytoplasmic staining was observed in neuro-2a cells.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SAH3 Antibody

  • adenosylhomocysteinase-like 2
  • AdoHcyase 3
  • ADOHCYASE3
  • EC 3.3.1.1
  • FLJ21719
  • KIAA0828S-adenosylhomocysteine hydrolase-like 2
  • putative adenosylhomocysteinase 3
  • S-adenosylhomocysteine hydrolase-like protein 2
  • S-adenosyl-L-homocysteine hydrolase 3

Background

In mammals, S-Adenosylhomocysteine hydrolase (SAH3 / AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, SAH3 is a regulator of biological transmethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AHCYL2
Entrez
Uniprot