RPS20 Antibody (SC65-08)

Western Blot: RPS20 Antibody (SC65-08) [NBP2-67628] - Analysis of RPS20 on different lysates with Rabbit anti-RPS20 antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: HUVEC cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 13 kDa Observed band size: 13 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: RPS20 Antibody (SC65-08) [NBP2-67628] - ICC staining RPS20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPS20 Antibody (SC65-08) [NBP2-67628] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-RPS20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: RPS20 Antibody (SC65-08) [NBP2-67628] - Analysis of RPS20 on different lysates using anti-RPS20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431

Immunocytochemistry/Immunofluorescence: RPS20 Antibody (SC65-08) [NBP2-67628] - ICC staining RPS20 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPS20 Antibody (SC65-08) [NBP2-67628] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RPS20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: RPS20 Antibody (SC65-08) [NBP2-67628] - Analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-RPS20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: RPS20 Antibody (SC65-08) [NBP2-67628] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-RPS20 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

RPS20 Antibody (SC65-08) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human RPS20 aa 36-80 / 119. (SwissProt: P60866 Human; SwissProt: P60867 Mouse; SwissProt: P60868 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RPS20
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RPS20 Antibody (SC65-08)

• 40S ribosomal protein S20
• FLJ27451
• MGC102930
• ribosomal protein S20

Background

Mammalian ribosomal proteins are encoded by multigene families that consist of processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein S20, also known as RPS20, is a 119 amino acid cytoplasmic protein that is a component of the 40S ribosomal subunit. Co-transcribed with the small nucleolar RNA gene U54, Ribosomal Protein S20 is a primary binding protein (it binds independently to its target protein) that interacts with both the 5' and 3' minor domains of 16S ribosomal RNA (rRNA). Through its interactions with 16S rRNA, Ribosomal Protein S20 is thought to play a key role in nucleating the assembly of the 30S ribosomal subunit. Like most ribosomal protein-coding genes, the gene encoding Ribosomal Protein S20 is dispersed throughout the genome and exists as multiple processed pseudogenes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS20