![Western Blot: RNPEPL1 Antibody (11G1) [NBP2-59373] - Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1 (SMC-265). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-265) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA.](http://images.novusbio.com/fullsize/RNPEPL1-Antibody-11G1-Western-Blot-NBP2-59373-img0001.jpg "Western Blot: RNPEPL1 Antibody (11G1) [NBP2-59373] - Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 11G1 (SMC-265). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (SMC-265) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 2 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 75kDa RDHKH-BSA, and REHKH-BSA.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB |
| Clone | 11G1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Mouse RNPEPL1 Antibody (11G1) - BSA Free (NBP2-59373) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Synthetic N-terminal arginylated peptide conjugated to KLH |
| Localization | Endoplasmic Reticulum |
| Specificity | Specific for N-terminal arginine, next to both glutamic acid and aspartic acid. Does not detect internal arginine. |
| Isotype | IgG3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNPEPL1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | RNPEPL1 |
