RNF180 Antibody (1C4) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RNF180 Antibody (1C4) [H00285671-M05] - Analysis of monoclonal antibody to RNF180 on HeLa cell. Antibody concentration 10 ug/ml

ELISA: RNF180 Antibody (1C4) [H00285671-M05] - Detection limit for recombinant GST tagged RNF180 is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RNF180 Antibody (1C4) - Azide and BSA Free Summary

• Immunogen: RNF180 (NP_848627, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKRSKELITKNHSQEETSILRCWKCRKCIASSGCFMEYLENQVIKDKDDSVDAQNICHVWHMNVEALPEWISCLIQKAQWTVGKLNCPFCGARLGGFNFV
• Specificity: RNF180 - ring finger protein 180 (1C4)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF180
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RNF180 Antibody (1C4) - Azide and BSA Free

• E3 ubiquitin-protein ligase RNF180
• EC 6.3.2.-
• MGC120328
• ring finger protein 180MGC120326

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RNF180 Antibody (H00285671-M05)(2)

Publications using H00285671-M05

• Zhang N, Gao X, Yuan Q et Al. E3 ubiquitin ligase RNF180 prevents excessive PCDH10 methylation to suppress the proliferation and metastasis of gastric cancer cells by promoting ubiquitination of DNMT1 Clin Epigenetics 2023-05-05 [PMID: 37147733]
• Wei F, Ba S, Jin M et al. RNF180 Inhibits Proliferation and Promotes Apoptosis of Colorectal Cancer Through Ubiquitination of WISP1 Frontiers in Cell and Developmental Biology 2021-01-22 [PMID: 33553163] (Immunocytochemistry/ Immunofluorescence)

Bioinformatics

• Gene Symbol: RNF180
• Entrez:
  • 285671(Human)
• Uniprot:
  • NP_848627(Human)