![Immunocytochemistry/Immunofluorescence: RNF180 Antibody (1C4) [H00285671-M05] - Analysis of monoclonal antibody to RNF180 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize2/RNF180-Antibody-(1C4)-Immunocytochemistry-Immunofluorescence-H00285671-M05-img0001.jpg "Immunocytochemistry/Immunofluorescence: RNF180 Antibody (1C4) [H00285671-M05] - Analysis of monoclonal antibody to RNF180 on HeLa cell. Antibody concentration 10 ug/ml")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 1C4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | RNF180 (NP_848627, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.MKRSKELITKNHSQEETSILRCWKCRKCIASSGCFMEYLENQVIKDKDDSVDAQNICHVWHMNVEALPEWISCLIQKAQWTVGKLNCPFCGARLGGFNFV |
| Specificity | RNF180 - ring finger protein 180 (1C4) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNF180 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
Diseases for RNF180 Antibody (H00285671-M05)Discover more about diseases related to RNF180 Antibody (H00285671-M05).
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PTMs for RNF180 Antibody (H00285671-M05)Learn more about PTMs related to RNF180 Antibody (H00285671-M05).
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![Sandwich ELISA: RNF180 Antibody (1C4) [H00285671-M05] - Detection limit for recombinant GST tagged RNF180 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/RNF180 Antibody (1C4)-Sandwich ELISA-H00285671-M05-img0002.jpg "Sandwich ELISA: RNF180 Antibody (1C4) [H00285671-M05] - Detection limit for recombinant GST tagged RNF180 is 3 ng/ml as a capture antibody.")
![Western Blot: 5-HT1A Antibody [NBP2-21590] - Sample (30 ug of whole cell lysate) A: Molt-4 B: Raji 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/5-HT1A-Antibody-Western-Blot-NBP2-21590-img0004.jpg)
![Immunohistochemistry-Paraffin: 5-HT1A Antibody [NBP2-21590] - Paraffin-embedded mouse duodenum. 5HT1A Receptor antibody [N3C1], Internal dilution: 1:500.](http://images.novusbio.com/fullsize2/5-HT1A-Antibody-Immunohistochemistry-Paraffin-NBP2-21590-img0006.jpg)
![Western Blot: 5-HT1A Antibody [NBP2-21590] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/5-HT1A-Antibody-Western-Blot-NBP2-21590-img0003.jpg)
![Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Annexin V Apoptosis Kit [NBP2-29373] - Adherent RAW cells were treated with 0.05 ug/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Accutase and stained with the Annexin V-FITC Apoptosis Detection Kit.](http://images.novusbio.com/fullsize2/Annexin-V-Apoptosis-Kit-[FITC]-NBP2-29373-img0006.jpg)
![Annexin V Apoptosis Kit [FITC] [NBP2-29373]](http://images.novusbio.com/fullsize2/Annexin-V-Apoptosis-Kit-[FITC]-NBP2-29373-img0001.jpg)
![Annexin V Apoptosis Kit [FITC] [NBP2-29373] - During early apoptosis, the plasma membrane loses asymmetry causing PS to be translocated from the cytoplasmic face of the plasma membrane to the external face which can be detected using Annexin V. As apoptosis progresses and the plasma membrance becomes comprised, PI is used as an additional marker of apoptosis.](http://images.novusbio.com/fullsize2/Annexin-V-Apoptosis-Kit-[FITC]-NBP2-29373-img0003.jpg)
![TIMP-3 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "TIMP-3 [Unconjugated]")
![Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.](http://images.novusbio.com/fullsize2/p19ARF-Antibody-Western-Blot-NB200-106-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.](http://images.novusbio.com/fullsize2/p19ARF-Antibody-Immunocytochemistry-Immunofluorescence-NB200-106-img0006.jpg)
![Immunohistochemistry-Paraffin: p19ARF Antibody [NB200-106] - IHC analysis of formalin fixed paraffin-embedded (FFPE) mouse spleen using p19ARF antibody at 10 ug/ml on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize2/p19ARF-Antibody-Immunohistochemistry-Paraffin-NB200-106-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]](http://images.novusbio.com/fullsize2/Insulin Antibody (E2-E3 (same as INS04))-SDS-Page-NBP2-34260-img0003.jpg)
![Western Blot: MTSS1 Antibody [NBP2-24716] - Analysis of MTSS1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat testis lysate using NBP2-24716 at 2 ug/ml, 4 ug/ml and 2 ug/ml, respectively.](http://images.novusbio.com/fullsize2/MTSS1-Antibody-Western-Blot-NBP2-24716-img0005.jpg)
![Immunohistochemistry-Paraffin: MTSS1 Antibody [NBP2-24716] - Analysis of human colon using this antibody at 10 ug/ml.](http://images.novusbio.com/fullsize2/MTSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-24716-img0004.jpg)
![Western Blot: ZNF645 Antibody [NBP2-21037] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize2/ZNF645 Antibody-Western Blot-NBP2-21037-img0001.jpg)
![Immunohistochemistry-Paraffin: ZNF645 Antibody [NBP2-21037] - Immunohistochemical analysis of paraffin-embedded GASTRIC CA, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/ZNF645 Antibody-Immunohistochemistry-Paraffin-NBP2-21037-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: UbcH6/UBE2E1 Antibody [NBP1-78983] - UBCH6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize2/UbcH6-UBE2E1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78983-img0005.jpg)
![Immunohistochemistry: UbcH6/UBE2E1 Antibody [NBP1-78983] - Analysis of UBCH6 in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/UbcH6-UBE2E1-Antibody-Immunohistochemistry-NBP1-78983-img0006.jpg)
![Western Blot: MUL1 Antibody [NBP2-31363] - Western blot analysis of K562 (A), Rt4 (B), and SH-SY5Y (C) cell lysate using MUL1 antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize2/MUL1-Antibody-Western-Blot-NBP2-31363-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: MUL1 Antibody [NBP2-31363] - MUL1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used.](http://images.novusbio.com/fullsize2/MUL1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31363-img0012.jpg)
![Western Blot: MUL1 Antibody [NBP2-31363] - WB analysis of MUL1 protein in lysate of human liver and partial recombinant MUL1 protein by using the primary antibody concentration of 2ug/ml and 0.5 ug/ml respectively.](http://images.novusbio.com/fullsize2/MUL1-Antibody-Western-Blot-NBP2-31363-img0001.jpg)
![Immunohistochemistry-Paraffin: TRIM50 Antibody [NBP1-81988] - Staining of human bone marrow shows strong cytoplasmic positivity in a subset of bone marrow poietic cells.](http://images.novusbio.com/fullsize2/TRIM50-Antibody-Immunohistochemistry-Paraffin-NBP1-81988-img0005.jpg)
