RGS1 Antibody

Product Discontinued

RGS1 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human RGS1. The immunogen region is: Internal.
• Specificity: RGS1 Antibody detects endogenous levels of total RGS1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RGS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25897806)
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RGS1 Antibody

• 1R20
• 1R20Early response protein 1R20
• B-cell activation protein BL34
• BL34
• Early response protein 1R20
• HEL-S-87
• IER1
• IR20
• IR20immediate-early response 1, B-cell specific
• regulator of G-protein signaling 1
• regulator of G-protein signalling 1
• RGS1

Background

This gene encodes a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RGS1 Antibody (NBP1-68645)(5)

Publications using NBP1-68645

• Hafez N, Refaat L, ElGebaly OK et al. Prognostic value of RGS1 and mTOR Immunohistochemical expression in Egyptian multiple myeloma patients; A single center study PloS one 2023-07-12 [PMID: 37437037] (IHC-P, Human)
  
  Details:
  1:250 IHC-P dilution
• Roh J, Shin SJ, Lee AN et al. RGS1 expression is associated with poor prognosis in multiple myeloma. J Clin Pathol 2016-07-21 [PMID: 27445341]
• Nemery E, Gabriel A, Piret J, Antoine N. Nociceptive and sympathetic innervations in the abaxial part of the cranial horn of the equine medial meniscus: an immunohistochemical approach. J Anat 2016-12-01 [PMID: 27345299] (IF/IHC, Human)
• Pak HK, Gil M, Lee Y et al. Regulator of G Protein Signaling 1 Suppresses CXCL12-Mediated Migration and AKT Activation in RPMI 8226 Human Plasmacytoma Cells and Plasmablasts PLoS ONE 2015-04-22 [PMID: 25897806] (ICC/IF, Human)
• Huen NY, Pang AL, Tucker JA et al. Up-regulation of proliferative and migratory genes in regulatory T cells from patients with metastatic castration-resistant prostate cancer. Int J Cancer 2013-01-15 [PMID: 23319273] (FLOW, Human)

Bioinformatics

• Gene Symbol: RGS1
• Entrez:
  • 5996(Human)
  • 50778(Mouse)
  • 54289(Rat)
• OMIM:
  • 600323(Human)
• Uniprot:
  • Q08116(Human)