Recombinant Rat Neurexophilin-1/NXPH1 Protein, CF

• Reactivity: Rt
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Rat Neurexophilin-1/NXPH1 Protein, CF Summary

• Details of Functionality: Measured in a competitive binding assay. When rrNeurexin-1 alpha (Catalog # 4485-NX) is immobilized at 1 µg/mL (100 µL/well), rrNeurexophilin-1 inhibits 50% binding of biotinylated rrNeurexophilin-1 (1 µg/mL) at the concentration range of 1-3 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived rat Neurexophilin-1 protein Ala22-Gly271 & Arg107-Gly271, both with a C-terminal 6-His tag
• Accession #: Q63366
• N-terminal Sequence: Ala22 & Arg107
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nxph1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 19 kDa & 29.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 27-30 kDa and 42-50 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Neurexophilin-1/NXPH1 Protein, CF

• neurexophilin 1
• Neurexophilin1
• Neurexophilin-1
• NPH1
• NXPH1

Background

Neurexophilin-1 (NXPH-1) is one of at least four vertebrate neuropeptide-like secreted glycoproteins in the neurexophilin family (1, 2). The 29 kDa, 271 amino acid (aa) NXPH-1 sequence contains a 22 aa signal peptide, a 94 aa propeptide that is cleaved at a basic motif, and a 115 aa mature protein that contains three potential N-glycosylation sites in the N-terminal portion and six conserved cysteines in the C-terminal portion (1). Mature rat NXPH-1 shares 99%, 99%, 99%, 96% and 84% aa identity with mouse, human, bovine, opossum and zebrafish NXPH-1, respectively. NXPH-1 is expressed selectively in subpopulations of neurons within the cerebral cortex, cerebellum and olfactory bulb that are thought likely to be inhibitory interneurons (2, 3). NXPH-3 is the neurexophilin most similar to NXPH-1, sharing 69% aa identity within the mature region. Expression of NXPH-1 and NXPH-3 does not appear to coincide, but both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 4, 5). Genetic deletion of NXPH-1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show defects in motor coordination (4, 6). Of the other known neurexophilins, NXPH-2 is not expressed in rodents, and NXPH-4 does not bind alpha -neurexins (1, 4).

1. Missler, M. and T. C. Sudhof (1998) J. Neurosci. 18:3630.
2. Petrenko, A. G. et al. (1996) J. Neurosci. 16:4360.
3. Clarris, H. J. et al. (2002) Int. J. Dev. Biol. 46:649.
4. Missler, M. et al. (1998) J. Biol. Chem. 273:34716.
5. Dudanova, I. et al. (2006) J. Neurosci. 26:10599.
6. Beglopoulos, V. et al. (2005) Mol. Cell. Biol. 25:7278.

Publications for Neurexophilin-1 (4208-NX)(1)

Publication using 4208-NX

• Kinzfogl J, Hangoc G, Broxmeyer HE Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells. Blood, 2011-05-31;118(3):565-75. 2011-05-31 [PMID: 21628410] (Bioassay, In Vivo, Human, Mouse)

Bioinformatics

• Gene Symbol: Nxph1
• Uniprot:
  • Q63366()