Recombinant Mouse Semaphorin 4F Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 4F Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to promote the survival of rat embryonic oligodendrocyte precursor cells. The cell survival is significantly enhanced at 3.0 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse Semaphorin 4F protein
  

  Mouse Semaphorin 4F (Arg41-Gly667) Accession # Q9Z123	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q9Z123
• N-terminal Sequence: Arg41
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sema4f
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 95.9 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 4F Fc Chimera Protein, CF

• M-SEMA
• m-Sema-M
• PRO2353
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F
• Sema M
• Sema W
• SEMA4F
• SEMAM
• Semaphorin 4F
• semaphorin-4F
• semaphorin-M
• semaphorin-W
• SEMAW
• SEMAWsemaphorin M

Background

Semaphorin 4F (Sema4F; previously called Sema W) is a 100-150 kDa member of the class 4 family of type I transmembrane semaphorins (1-3). Mature mouse Sema4F is a 737 amino acid (aa) protein that contains a Sema domain, a PSI region, and a C2-type Ig-like domain within its extracellular domain (ECD; aa 41-667) (2). Within the ECD, mouse Sema4F shares 92% and 98% aa identity with human and rat Sema4F, respectively. Sema4F is expressed by cell types that include Schwann cells, oligodendrocyte (highest in precursors), retinal ganglion cells, and neurons, especially along migratory pathways (2-4). It is found within post-synaptic densities at glutamatergic synapses, where it binds and probably recruits the cytoplasmic scaffolding protein PSD-95 (3, 5). In Schwann cells, Sema4F likely acts as an adhesive protein and a mediator of contact inhibition, and thus an inhibitor of cell proliferation (6). In neurofibromatosis type 1 (NT1), loss of the NF1 protein results in loss of Schwann cell Sema4F expression via perturbation of ERK signals (6). Loss of Sema4F in turn disrupts adhesive interaction and alignment of Schwann cells with neurons (6). For oligodendroglial precursors in culture, immobilized Sema4F inhibits migration and enhances survival, differentiation and adhesion (3). A secreted form of approximately 80 kDa is reported in prostate cancer cells, transfected cell culture supernatants, and adult brain lysates (3, 7).

1. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
2. Encinas, J.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2491.
3. Armendariz, B.G. et al. (2012) Mol. Cell. Neurosci. 49:54.
4. Cohen, R.I. et al. (2003) J. Neurochem. 85:1262.
5. Schultze, W. et al. (2001) J. Neurochem. 78:482.
6. Parrinello, S. et al. (2008) Genes Dev. 22:3335.
7. Ayala, G.E. et al. (2008) Clin. Cancer Res. 14:7593.

Bioinformatics

• Gene Symbol: Sema4f
• Uniprot:
  • Q9Z123(Mouse)