Recombinant Mouse IFN-alpha 9 Protein, CF

Recombinant Mouse IFN-alpha 9 (10369-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 100 pg/mL.

2 μg/lane of Recombinant Mouse IFN-alpha 9 (Catalog # 10369-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IFN-alpha 9 Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is < 100 pg/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 9 protein
  Cys24-Glu190
• Accession #: P09235.1
• N-terminal Sequence: Cys24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW:

  19 kDa

  .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18-24 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-alpha 9 Protein, CF

• IFA9
• IFNA9
• IFN-alpha 9

Background

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). The extracellular domain (ECD) of mature mouse IFNA9 consists of 166 amino acids (aa), with the closest homolog identified in humans as IFNA1. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection. In gB DNA-Vaccinated mice, coimmunisation with IFNA9 has been shown to enhance protective immunity against cytomegalovirus and myocarditis (5).

1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
4. van Pesch, V. et al. (2004) J. Virol. 78:8219.
5. James C.M. et al. (2007) Vaccine. 25(10):1856.
6. Cull, V.S. et al. (2002) Gene Therapy. 9:1369-78.

Bioinformatics

• Uniprot:
  • P09235.1()