Recombinant Mouse CD200R4 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD200R4 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 R4 is present at 0.5 μg/mL, Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) binds with a typical ED₅₀ of 0.8-4 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD200 R4 protein
  Thr26-Thr241, with a C-terminal 6-His tag
• Accession #: Q6XJV4
• N-terminal Sequence: Thr26
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 49-74 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD200R4 Protein, CF

• CD200 R4
• CD200R4
• F630107N04Rik
• MCD200
• MCD200RLa

Background

CD200 R4, also known as CD200RLa, is a transmembrane protein in the immunoglobulin superfamily (1). Mature mouse CD200 R4 consists of a 216 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and an 8 aa cytoplasmic domain (2, 3). The ECD contains one Ig-like V-type domain and one Ig-like
C2-type domain. A positively charged lysine in the transmembrane likely mediates association with the signaling molecule DAP12 (2). CD200 R4 is expressed on monocytes, bone marrow derived macrophages and dendritic cells, NK cells, and resting mast cells. It is down-regulated following Fc epsilon  RI-mediated mast cell activation (2). According to conflicting reports, CD200 R4 may or may not interact with CD200 (3, 4). R&D Systems in-house testing indicates that this recombinant mouse CD200 R4 binds to recombinant mouse CD200-Fc.

1. Holmannova, D. et al. (2012) Acta Medica (Hradec Kralove) 55:12.
2. Wright, G.J. et al. (2003) J. Immunol. 171:3034.
3. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
4. Hatherley, D. et al. (2005) J. Immunol. 175:2469.

Bioinformatics

• Uniprot:
  • Q6XJV4()