Recombinant Mouse CD200R1L Fc Chimera Protein, CF

When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 μg/mL (100 μL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED50 of 0.35-2.8 μg/mL.

2 μg/lane of Recombinant Mouse CD200R1L Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 65-85 kDa and 130-170 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD200R1L Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 Fc Chimera (Catalog # 3355-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Mouse CD200R1L Fc Chimera (Catalog # 10198-CD) binds with an ED₅₀ of 0.35-2.8 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD200R1L protein
  

  Mouse CD200R1L (Ile25-Thr220) Accession # Q6XJV6.1	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q6XJV6.1
• N-terminal Sequence: Ile25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-85 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD200R1L Fc Chimera Protein, CF

• cell surface glycoprotein CD200 receptor 2
• CD200 receptor 1-like
• CD200R1L
• CD200R2
• CD200RLa

Background

CD200 Receptor 2 (CD200R2), or CD200R1L, is a type I transmembrane receptor for the CD200 cell surface glycoprotein. Mature CD200R2 consists of an extracellular domain (ECD) containing an Ig-like V-type domain and an Ig-like C2-type domain, a transmembrane segment and a short cytoplasmic domain. The ECD of mouse CD200R2 shares a 76% and 63% amino acid (aa) identity with the rat and human ECD, respectively. CD200R2 is strongly expressed on resting mast cells but deceased upon activation via Fc epsilon R1. It is also observed on monocytes, bone marrow-derived dendritic cells and TH2 cells (1, 2, 4). It has been demonstrated CD200R2 can interact with CD200 (3) and signal through adaptor protein, DAP12, via its lysine residue in the transmembrane region (2). In addition, it has been reported CD200 and CD200R2 interaction alters dendritic cell differentiation and enhances induction of CD4⁺CD25⁺Foxp3⁺ regulatory T cells in mouse transplant model (5).

1. Wright, G. et al. (2003) J. Immunol. 171:3034.
2. Voehringer, D. (2004) J. Biol. Chem. 279(52):54117.
3. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
4. Minas, K. (2006) Crit Rev Immunol 26(3):213.
5. Gorczynski, R. et al. (2008) J. Immunol. 180(9):5946.

Bioinformatics

• Uniprot:
  • Q6XJV6.1()