Recombinant Human Soggy-1/DkkL1 Protein

Images

 
There are currently no images for Soggy-1/DkkL1 (1549-S1).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Soggy-1/DkkL1 Protein Summary

Details of Functionality
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is 2-6 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Soggy-1/DkkL1 protein
Ala31-Leu242, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ala31
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Gene
DKKL1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
25.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa, reducing conditions
Publications
Read Publications using
1549-S1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Soggy-1/DkkL1 Protein

  • Cancer/testis antigen 34
  • CT34dickkopf-like 1 (soggy)
  • dickkopf-like 1
  • DkkL1
  • Protein soggy-1
  • SGY
  • SGY1
  • SGY-1dickkopf-like protein 1
  • SGY1soggy
  • Soggy1
  • Soggy-1

Background

Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The human Soggy-1 cDNA encodes a 242 amino acid (aa) precursor that includes a 30 aa signal sequence (2). Human Soggy-1 shares approximately 65% aa sequence identity with mouse and rat Soggy-1 and 15 - 20% aa sequence identity with human Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3 - 5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).

  1. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  2. Krupnik, V.E. et al. (1999) Gene 238:301.
  3. Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
  4. Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
  5. Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
  6. Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.

Customers Who Viewed This Item Also Viewed...

NBP2-24613
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NBP2-55789
Species: Hu
Applications: ICC/IF
NBP1-82598
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-80524
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
AF1647
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
6507-IL/CF
Species: Hu
NBP2-37510
Species: Hu
Applications: WB, ELISA, Flow, CyTOF-ready
AF1505
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP1-92364
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-01151
Species: Hu
Applications: WB, Flow, IHC, IHC-P
H00006202-Q01
Species: Hu
AF1118
Species: Hu
Applications: WB, IHC
AF2435
Species: Mu
Applications: WB, IHC
1269-DK/CF
Species: Hu
NBP2-02031
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P
NBP2-15432
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
1549-S1
Species: Hu
Applications: Bioactivity

Publications for Soggy-1/DkkL1 (1549-S1)(3)

Reviews for Soggy-1/DkkL1 (1549-S1) (0)

There are no reviews for Soggy-1/DkkL1 (1549-S1). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Soggy-1/DkkL1 (1549-S1) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Soggy-1/DkkL1 Products

Bioinformatics Tool for Soggy-1/DkkL1 (1549-S1)

Discover related pathways, diseases and genes to Soggy-1/DkkL1 (1549-S1). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Soggy-1/DkkL1 (1549-S1)

Discover more about diseases related to Soggy-1/DkkL1 (1549-S1).
 

Pathways for Soggy-1/DkkL1 (1549-S1)

View related products by pathway.

PTMs for Soggy-1/DkkL1 (1549-S1)

Learn more about PTMs related to Soggy-1/DkkL1 (1549-S1).

Blogs on Soggy-1/DkkL1

There are no specific blogs for Soggy-1/DkkL1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Soggy-1/DkkL1 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol DKKL1
Uniprot
COVID-19 update