Recombinant Human Semaphorin 4F Fc Chimera Protein, CF

2 μg/lane of Recombinant Human Semaphorin 4F Fc Chimera (Catalog # 10113-S4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 96-111 kDa and 190-220 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Semaphorin 4F Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 4F protein

  Human Semaphorin 4F (Arg35-Val659) Accession # O95754-1

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: O95754-1
• N-terminal Sequence: Arg35
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 96-111 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in Water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 4F Fc Chimera Protein, CF

• M-SEMA
• m-Sema-M
• PRO2353
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4F
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4F
• Sema M
• Sema W
• SEMA4F
• SEMAM
• Semaphorin 4F
• semaphorin-4F
• semaphorin-M
• semaphorin-W
• SEMAW
• SEMAWsemaphorin M

Background

Semaphorin 4F (Sema4F; previously called Sema W) is a 100-150 kDa member of the class 4 family of type I transmembrane semaphorins (1-3). It is expressed in Schwann cells, oligodendrocyte (highest in precursors), retinal ganglion cells, and neurons, especially along migratory pathways (2-4). It is found within post-synaptic densities at glutamatergic synapses, where it binds and probably recruits the cytoplasmic scaffolding protein PSD-95 (3, 5). There are two isoforms of human Sema4F. The short isoform is missing aa 120-274. The canonical long isoform is synthesized as a 770 aa protein that includes a 34 aa signal peptide, a 625 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 90 aa cytoplasmic tail. The ECD contains a Sema domain, a PSI region, and a C2-type Ig-like domain. Within the ECD, human Sema4F shares 92% aa sequence identity with the mouse and rat Sema4F. Sema4F is expressed at the highest levels postnatally suggesting an important role in nerve system maintenance and repair (2). In Schwann cells, Sema4F acts as an adhesive protein and a mediator of contact inhibition, and thus an inhibitor of Schwann cell proliferation (6). In neurofibromatosis type 1 (NT1), loss of the NF1 protein results in loss of Schwann cell Sema4F expression via perturbation of ERK signals (6). Loss of Sema4F in turn disrupts adhesive interaction and alignment of Schwann cells with neurons (6). For oligodendroglial precursors in culture, immobilized Sema4F inhibits migration and enhances survival, differentiation and adhesion (3). Sema4F is involved in cancer-induced neurogenesis and may serve as a critical regulator of neuro-epithelial interactions and a biomarker of aggressive prostate cancer (7).

1. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
2. Encinas, J.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2491.
3. Armendariz, B.G. et al. (2012) Mol. Cell. Neurosci. 49:54.
4. Cohen, R.I. et al. (2003) J. Neurochem. 85:1262.
5. Schultze, W. et al. (2001) J. Neurochem. 78:482.
6. Parrinello, S. et al. (2008) Genes Dev. 22:3335.
7. Ding, Y. et al. (2013) Clin. Cancer Res. 19:6101.

Bioinformatics

• Uniprot:
  • O95754-1()