Recombinant Human NETO2 His-tag Protein, CF

2 μg/lane of Recombinant Human NETO2 His-tag Protein (Catalog # 10975-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 32-45 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human NETO2 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human NETO2 protein
  Ile23-Lys345, with a C-terminal 6-His tag
• Accession #: Q8NC67.1
• N-terminal Sequence: Ile23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 32-45 kDa, reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NETO2 His-tag Protein, CF

• Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor classA domains protein 2
• BTCL2
• FLJ10430
• FLJ90456
• NEOT2
• NEOT2FLJ14724
• NETO2
• neuropilin (NRP) and tolloid (TLL)-like 2
• neuropilin and tolloid-like protein 2

Background

NETO2, or Neuropilin and tolloid-like protein 2, is a type 1 transmembrane protein belonging to the CUB (for complement C1r/C1s, Ueqf, Bmp1) domain and LDLa‑containing protein family that modulates the neuronal kainate receptors (KARs) (1-3). It is mainly expressed in neural tissues but was also reported to be expressed in several non-neural tissues and its expression has been associated with various cancers such as renal, lung, colon, cervical and colorectal cancers (4-6). Mature human NETO2 is composed of a 325 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 157 aa cytoplasmic region. Within the ECD, human NETO2 shares 98% aa identity with mouse and rat NETO2. It functions as an accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3 regulating channel activities and synaptic transmission (7).

1. Fedorova, M.S. et al. (2020) Front. Genet. 11:594933. doi: 10.3389/fgene.2020.594933.
2. Stohr, H. et al. (2002) Gene 286:223.
3. Straub, C. et al. (2011) J. Neurosci. 31:8078.
4. Li, Y. et al. (2019) Cancer Manag. Res. 11:5147.
5. Niu, O. et al. (2012) Genetika. 48:506.
6. Xu, J. et al. (2021) Int. J. Biol. Sci. 17:259.
7. Griffith T. et al. (2015) J. Physiol. 593:4815.

Bioinformatics

• Uniprot:
  • Q8NC67.1()