Recombinant Human Lipocalin-1 Protein, CF

Images

 
There are currently no images for Lipocalin-1 (1708-PI).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Inhibition Activity
Format
Carrier-Free

Order Details

Recombinant Human Lipocalin-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008). The IC50 value is <400 nM, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human Lipocalin-1 protein
His19-Asp176, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
His19
Protein/Peptide Type
Recombinant Enzymes
Gene
LCN1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-22 kDa, reducing conditions
Publications
Read Publication using
1708-PI in the following applications:

  • MS
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 50 mM Sodium Acetate, 0.15 M NaCl, pH 5.5
  • Cathepsin Buffer: 50 mM Sodium Acetate, 0.15 M NaCl, 10 mM DTT, pH 5.5
  • Substrate Buffer: 50 mM Sodium Acetate, 0.15 M NaCl, 5 mM DTT, pH 5.5
  • Recombinant Human Lipocalin-1 (rhLipocalin-1) (Catalog # 1708-PI)
  • Recombinant Human Cathepsin V (rhCathepsin V) (Catalog # 1080-CY)
  • Substrate: Z-Leu-Arg-AMC (Catalog # ES008)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Prepare a curve of rhLipocalin-1 (MW: 18,805 Da) in Assay Buffer. Make the following serial dilutions:  40,000, 10,000, 4000, 1000, 500, 250, 125, 50, 12.5, and 6.25 nM.
  2. Dilute rhCathepsin V to 2 µg/mL in Cathepsin Buffer.
  3. Mix equal volumes of the rhLipocalin-1 curve dilutions and the 2 µg/mL rhCathepsin V. Include a Cathepsin Control (in duplicate) containing Assay Buffer in place of rhLipocalin-1.
  4. Incubate mixtures at room temperature for 30 minutes.
  5. Dilute Substrate to 40 µM in Substrate Buffer.
  6. Load into a black well plate 50 µL of the incubated mixtures, and start the reaction by adding 50 µL of 40 µM Substrate.
  7. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes.
  8. Derive the 50 % inhibiting concentration (IC50) of rhLipocalin-1 by plotting RFU/min (or specific activity) vs. concentration with 4‑PL fitting.
  9. The specific activity for rhCathepsin V at each point may be determined using the following formula (if needed):

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).

Per Well:
  • rhCathepsin V: 0.05 µg
  • rhLipocalin-1: 10,000, 2500, 1000, 250, 125, 62.5, 31.25, 12.5, 3.125, and 1.5625 nM
  • Substrate: 20 µM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lipocalin-1 Protein, CF

  • LCN1
  • lipocalin 1 (tear prealbumin)
  • lipocalin 1-like 2
  • Lipocalin1
  • Lipocalin-1
  • MGC71975
  • PMFA
  • Tear lipocalin
  • Tear Prealbumin
  • TLC
  • TPVon Ebner gland protein
  • VEG protein
  • VEGP
  • VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin)

Background

Lipocalin-1, also known as tear prealbumin or von Ebner’s gland protein (VEGP), is encoded by the LCN1 gene (1-3). It is a member of the Lipocalin superfamily that binds many different classes of lipophylic chemicals (4). Lipocalin-1 contains three sequence motifs similar to the cystatins, a superfamily of cysteine protease inhibitors (5). In fact, it has been suggested that Lipocalin-1 is a physiological inhibitor of cysteine proteases and plays a role in the control of inflammatory processes in oral and ocular tissues (5). Recombinant Human Lipocalin-1 corresponds to the mature and secreted protein. It is a weak inhibitor of cysteine proteases such as cathepsin V, which is similar to recombinant human Cystatin S (Catalog # 1296-PI).
  1. Redl, B. et al. (1992) J. Biol. Chem. 267:20282.
  2. Blaker, M. et al. (1993) Biochim. Biophys. Acta 1172:131.
  3. Lassagne, H. and A.M. Gachon (1993) Exp. Eye Res. 56:605.
  4. Redl, B. et al. (2000) Biochim. Biophys. Acta 1482:241.
  5. van’t Hof, W. et al. (1997) J. Biol. Chem. 272:1837.

Customers Who Viewed This Item Also Viewed...

NBP1-84916
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
H00001806-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NB110-68136
Species: Hu, Mu, Rt, Fe
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
NBP2-75442
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-02710
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-22439
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-20383
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NLS1049
Species: Hu, Eq, Mk, Pm
Applications: IHC-P, ICC
NBP1-71688
Species: Hu, Mu, Po
Applications: WB, Simple Western, ICC/IF
NBP1-85467
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-49879
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-32421
Species: Hu, Mu
Applications: WB, IHC, IHC-P
MAB29051
Species: Hu
Applications: IP, CyTOF-ready, ICC, ICFlow
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P

Publications for Lipocalin-1 (1708-PI)(1)

We have publications tested in 1 confirmed species: N/A.

We have publications tested in 1 application: MS.


Filter By Application
MS
(1)
All Applications
Filter By Species
N/A
(1)
All Species

Reviews for Lipocalin-1 (1708-PI) (0)

There are no reviews for Lipocalin-1 (1708-PI). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Lipocalin-1 (1708-PI) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Lipocalin-1 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol LCN1
Entrez
Uniprot