Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF

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When Recombinant Human LILRB5/CD85c/LIR-8 is coated at 2μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an apparent Kd <4 nM.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB5/CD85c/LIR-8 is coated at 2 μg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an apparent Kd <4 nM.
Source
Mouse myeloma cell line, NS0-derived human LILRB5/CD85c/LIR-8 protein
Arg18-His456, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg18
Protein/Peptide Type
Recombinant Proteins
Gene
LILRB5
Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
64-77 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LILRB5/CD85c/LIR-8 Protein, CF

  • CD85 antigen-like family member C
  • CD85c antigen
  • CD85c
  • Leukocyte immunoglobulin-like receptor 8
  • leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
  • LILRB5
  • LIR8
  • LIR-8
  • LIR8CD85C
  • LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5
  • member 5

Background

LIR-8, also known as CD85c and LILRB5, belongs to a family of transmembrane glycoproteins that negatively regulate immune cell activation (1). Mature human
LIR-8 consists of a 435 amino acid (aa) extracellular domain with four Ig-like domains, a 21 aa transmembrane segment, and a 111 aa cytoplasmic domain with two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (2). Alternative splicing of human LIR-8 generates an isoform that lacks the second Ig-like domain. LIR-8 is expressed on NK cells and in the tryptic granules of mast cells (2, 3). Following cell activation and degranulation, it is present on the mast cell surface (3). Activated mast cells may also release soluble forms of LIR-8 (3). Consistent with the demonstrated binding of LILRB2 to Angiopoietin-like 2 and 5 (4), R&D Systems in-house testing indicates that LIR-8 binds to Angiopoietin-like 7.
  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (1997) J. Immunol. 159:5192.
  3. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  4. Zheng, J. et al. (2012) Nature 485:656.

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Bioinformatics

Gene Symbol LILRB5
Entrez
Uniprot