Interferon was titrated with the use of the cytopathic effect inhibition assay as
described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons,"
J. Virol. 37, 755-758;
Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for
Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral
assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. As there
is no international standard for human interferon omega, the units are determined with respect to the international
reference standard for human interferon alpha A (Hu-IFN-
alpha A) provided by the National Institutes of Health [see Pestka, S.
(1986) "Interferon Standards and General Abbreviations," in
Methods in Enzymology (S. Pestka, ed.), Academic Press,
New York 119, 14-23]. Unit of activity measured on human lung carcinoma cell line A549 with encephalomyocarditis virus
<1.0 EU per 1 μg of the protein by the LAL method.
20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using 11395-1 in the following applications:
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Phosphate buffered saline (PBS) containing 2% bovine serum albumin (BSA)
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human IFN-omega Protein
IFN-omega 1, interferon omega-1
interferon, omega 1
Interferon-omega (IFN-omega), a type I interferon, is a monomeric glycoprotein distantly related in structure to IFN-alpha and IFN-beta, but unrelated to IFN-gamma. The human type I interferon receptor complex, which mediates the biological activity of IFN-alpha and IFN-beta, also binds IFN-omega. The complex is composed of a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand binding and signal transduction. IFN-omega is secreted by virus-infected leukocytes.
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