Recombinant Human IFN-omega Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IFN-omega Protein Summary

Details of Functionality
Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. As there is no international standard for human interferon omega, the units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN- alpha A) provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. Unit of activity measured on human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV).
Source
E. coli-derived human IFN-omega protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
IFNW1
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
11395-1 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Phosphate buffered saline (PBS) containing 2% bovine serum albumin (BSA)

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-omega Protein

  • IFN-omega 1, interferon omega-1
  • IFNomega
  • IFN-omega
  • IFNW1
  • Interferon alpha-II-1
  • interferon omega-1
  • interferon, omega 1

Background

Interferon-omega (IFN-omega), a type I interferon, is a monomeric glycoprotein distantly related in structure to IFN-alpha and IFN-beta, but unrelated to IFN-gamma. The human type I interferon receptor complex, which mediates the biological activity of IFN-alpha and IFN-beta, also binds IFN-omega. The complex is composed of a 100 kDa ligand-binding subunit (IFN-alpha R1) and a 125 kDa subunit (IFN-alpha R2) that is involved both in ligand binding and signal transduction. IFN-omega is secreted by virus-infected leukocytes.

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Publications for IFN-omega/IFNW1 (11395-1)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol IFNW1
Uniprot