Recombinant Human GPR115 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human GPR115 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U‑87 MG human glioblastoma/astrocytoma cells. When 5 x 10⁴ cells per well are added to rhGPR-115 coated plates (5 μg/mL, 100 μL/well), approximately 60-85% will adhere after 60 minutes at 37 °C.
• Source: Chinese Hamster Ovary cell line, CHO-derived human GPR115 protein
  Ser22-Ala347, with a C-terminal 6-His tag
• Accession #: Q8IZF3
• N-terminal Sequence: Ser22
• Protein/Peptide Type: Recombinant Proteins
• Gene: ADGRF4
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 66-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GPR115 Protein, CF

• FLJ38076
• G protein-coupled receptor 115
• GPR115
• G-protein coupled receptor PGR18
• PGR18
• PGR18G-protein coupled receptor 115
• probable G-protein coupled receptor 115
• seven transmembrane helix receptor

Background

GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N-terminal extracellular domain (ECD), seven TM regions separated by 6 - 24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3 - 7). Within the N terminal region that ends with the predicted cleavage site (aa 22 - 347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).

1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
3. Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.
4. Moriguchi, T. et al. (2004) Genes Cells 9:549.
5. Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.
6. Krasnoperov, V.G. et al. (1997) Neuron 18:925.
7. Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.

Bioinformatics

• Gene Symbol: ADGRF4
• Uniprot:
  • Q8IZF3(Human)