Recombinant Human GPR114 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human GPR114 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance the adhesion of T98G human glioblastoma cells to human Fibronectin. When 4 x 10⁴ cells/well are added to plates coated with human Fibronectin (0.1 µg/mL, Catalog # 1918-FN) and rhGPR114 (10 µg/well, 100 µL/well) for 60 minutes at 37° C, there is a 2-4 fold increase in adhesion to human fibronectin induced by the presence of rhGPR114.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived human GPR114 protein
  Glu22-Gly184 , with a C-terminal 6-His tag
• Accession #: Q8IZF4
• N-terminal Sequence: Glu22
• Protein/Peptide Type: Recombinant Proteins
• Gene: ADGRG5
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GPR114 Protein, CF

• G protein-coupled receptor 114
• G protein-coupled receptor PGR27
• GPR114
• G-protein coupled receptor PGR27
• PGR27
• PGR27G-protein coupled receptor 114
• probable G-protein coupled receptor 114

Background

GPR114 is a member of the LN-TM7 family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that exhibit long extracellular N-termini (1 - 3). The 528 amino acid (aa) human GPR114 sequence predicts a 21 aa signal sequence, a 229 aa N-terminal extracellular domain (ECD) and seven TM regions separated by short intracellular and extracellular regions. Like other LN-TM7 members, the ECD contains a highly glycosylated mucin-like stalk that is predicted to function in adhesion, followed by a GPCR proteolytic cleavage site (GPS) (2); it does not contain any other easily recognizable domains. Adhesion ligands have been reported for only a few LN-TM7 members (1). GPR114 is not one of them. Human GPR114 aa 22 - 184 share 63%, 63%, 67% and 59% aa identity with the corresponding regions of mouse, rat, bovine and equine GPR114, respectively. GPR114 was identified from expressed sequence tags (ESTs) found in leukemia cells, leukocytes, and pancreatic islets cells (2). An alternative start site has also been reported at Met 233 (4). One functional splice variant of GPR114 has been identified in colon, leukocytes, spleen and thymus (3).

1. Bjarnadottir, T.K. et al. (2007) Cell. Mol. Life Sci. 64:2104.
2. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
3. Bjarnadottir, T.K. et al. (2007) Gene 387:38.
4. Accession # EAW82937.

Bioinformatics

• Gene Symbol: ADGRG5
• Uniprot:
  • Q8IZF4()