Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

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Immobilized Recombinant Human Desmoglein‑4 Fc Chimera (Catalog #9230-DM) supports the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Desmoglein-4 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Desmoglein-4 protein
Human Desmoglein-4
(Glu24-Ala631)
Accession # Q86SJ6
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu24
Structure / Form
Disulfide-liked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
95-115 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

  • Cad13
  • Cadherin family member 13
  • CDGF13
  • CDHF13
  • CDHF13CDH family member 13
  • desmoglein 4
  • Desmoglein4
  • Desmoglein-4
  • DSG4
  • LAHdesmoglein-4

Background

Desmoglein-4 is an approximately 140 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-4 is synthesized with a 26 amino acid (aa) propeptide followed by a 582 aa extracellular domain (ECD) with four tandem cadherin-like domains, a 21 aa transmembrane segment, and a 388 aa cytoplasmic domain (1, 2). Within the ECD, human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4. Alternative splicing generates additional isoform with a substitution in the cytoplasmic domain. Desmoglein-4 is the principal cadherin of desmosomes which form adhesive contacts between epithelial cells (2, 3). It is expressed in hair follicles and differentiated keratinocytes in suprabasal layers of the skin and plays a role in keratinocyte differentiation and adhesion (2, 3). Mutations in human Desmoglein-4 are associated with the development of monilethrix autosomal recessive hypotrichosis (LAH) (2, 4-8). Autoantibodies directed against Desmoglein-3 in the blistering skin disorder pemphigus vulgaris can cross-react with Desmoglein-4 (2, 9, 10).
  1. Whittock, N.V. and C. Bower (2003) J. Invest. Dermatol. 120:523.
  2. Kljuic, A. et al. (2003) Cell 113:249.
  3. Bazzi, H. et al. (2006) Differentiation 74:129.
  4. Bazzi, H. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:222.
  5. Schaffer, J.V. et al. (2006) J. Invest. Dermatol. 126:1286.
  6. Shimomura, Y. et al. (2006) J. Invest. Dermatol. 126:1281.
  7. Zlotogorski, A. et al. (2006) J. Invest. Dermatol. 126:1292.
  8. Kato, M. et al. (2015) J. Invest. Dermatol. 135:1253.
  9. Nagasaka, T. et al. (2004) J. Clin. Invest. 114:1484.
  10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.

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