Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

Immobilized Recombinant Human Desmoglein‑4 Fc Chimera (Catalog #9230-DM) supports the adhesion of A431 human epithelial carcinoma cells. The ED50 for this effect is 0.4-2.4 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Desmoglein-4 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. The ED 50 for this effect is 0.4-2.4 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Desmoglein-4 protein

  Human Desmoglein-4 (Glu24-Ala631) Accession # Q86SJ6

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: Q86SJ6
• N-terminal Sequence: Glu24
• Structure / Form: Disulfide-liked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Desmoglein-4 Fc Chimera Protein, CF

• Cad13
• Cadherin family member 13
• CDGF13
• CDHF13
• CDHF13CDH family member 13
• desmoglein 4
• Desmoglein4
• Desmoglein-4
• DSG4
• LAHdesmoglein-4

Background

Desmoglein-4 is an approximately 140 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. Human Desmoglein-4 is synthesized with a 26 amino acid (aa) propeptide followed by a 582 aa extracellular domain (ECD) with four tandem cadherin-like domains, a 21 aa transmembrane segment, and a 388 aa cytoplasmic domain (1, 2). Within the ECD, human Desmoglein-4 shares 82% aa sequence identity with mouse and rat Desmoglein-4. Alternative splicing generates additional isoform with a substitution in the cytoplasmic domain. Desmoglein-4 is the principal cadherin of desmosomes which form adhesive contacts between epithelial cells (2, 3). It is expressed in hair follicles and differentiated keratinocytes in suprabasal layers of the skin and plays a role in keratinocyte differentiation and adhesion (2, 3). Mutations in human Desmoglein-4 are associated with the development of monilethrix autosomal recessive hypotrichosis (LAH) (2, 4-8). Autoantibodies directed against Desmoglein-3 in the blistering skin disorder pemphigus vulgaris can cross-react with Desmoglein-4 (2, 9, 10).

1. Whittock, N.V. and C. Bower (2003) J. Invest. Dermatol. 120:523.
2. Kljuic, A. et al. (2003) Cell 113:249.
3. Bazzi, H. et al. (2006) Differentiation 74:129.
4. Bazzi, H. et al. (2005) J. Investig. Dermatol. Symp. Proc. 10:222.
5. Schaffer, J.V. et al. (2006) J. Invest. Dermatol. 126:1286.
6. Shimomura, Y. et al. (2006) J. Invest. Dermatol. 126:1281.
7. Zlotogorski, A. et al. (2006) J. Invest. Dermatol. 126:1292.
8. Kato, M. et al. (2015) J. Invest. Dermatol. 135:1253.
9. Nagasaka, T. et al. (2004) J. Clin. Invest. 114:1484.
10. Calkins, C.C. et al. (2006) J. Biol. Chem. 281:7623.

Bioinformatics

• Uniprot:
  • Q86SJ6(Human)