Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

When Recombinant Human CD320/TCblR/8D6A Fc Chimera (Catalog # 1557‑CD) is coated at 25 ng/mL, Recombinant Human Transcobalamin II (Catalog # 7895‑TC) binds with an ED50 of 0.5-3 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human CD320/TCblR/8D6A Fc Chimera is immobilized at 25 ng/mL (100 μL/well), the concentration of Recombinant Human Transcobalamin II (Catalog # 7895-TC) that produces 50% of the optimal binding response is 0.5-3 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human CD320/TCblR/8D6A protein
  

  Human CD320 (Leu31-Val231) Accession # Q9NPF0	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9NPF0
• N-terminal Sequence: Leu31 and Ser36
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CD320
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD320/TCblR/8D6A Fc Chimera Protein, CF

• 8D6 antigen
• 8D6A
• 8D6AFDC-SM-8D6
• CD320 antigenFDC-signaling molecule 8D6,8D6TCBLR
• CD320 molecule
• CD320
• TCblR
• Transcobalamin receptor

Background

CD320, also known as the Transcobalamin II receptor (TCb1R) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature human CD320 consists of a 199 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2). Within the extracellular domain, human CD320 shares 52% aa sequence identity with mouse and rat CD320. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B₁₂) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a co‑stimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).

1. May, P. et al. (2007) Ann. Med. 39:219.
2. Li, L. et al. (2000) J. Exp. Med. 191:1077.
3. Quadros, E.V. et al. (2009) Blood 113:186.
4. Bose, S. et al. (1995) J. Biol. Chem. 270:8152.
5. Park, H.J. et al. (2009) Immune Netw. 9:138.
6. Cho, W. et al. (2008) BMC Rep. 41:863.
7. Zhang, X. et al. (2001) J. Immunol. 167:49.
8. Park, C.-S. and Y.S. Choi (2005) Immunology 114:2.
9. Li, L. et al. (2004) Blood 104:815.

Bioinformatics

• Gene Symbol: CD320
• Uniprot:
  • Q9NPF0()