RAMP3 Overexpression Lysate (Native)

Images

 
Western Blot: RAMP3 Overexpression Lysate (Adult Normal) [NBL1-15138] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAMP3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RAMP3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC208762 and based on accession number NM_005856. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens receptor (G protein-coupled) activity modifying protein 3 (RAMP3), mRNA.
Gene
RAMP3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RAMP3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RAMP3 Overexpression Lysate (Native)

  • calcitonin receptor-like receptor activity modifying protein 3
  • Calcitonin-receptor-like receptor activity-modifying protein 3
  • CRLR activity-modifying protein 3
  • RAMP3
  • receptor (calcitonin) activity modifying protein 3
  • receptor (G protein-coupled) activity modifying protein 3
  • receptor activity modifying protein 3
  • receptor activity-modifying protein 3
  • receptor-activity-modifying protein 3

Background

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP3) protein, CRLR functions as an adrenomedullin receptor. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for RAMP3 Lysate (NBL1-15138) (0)

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Bioinformatics

Gene Symbol RAMP3
Entrez