RAMP1 Antibody

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Western Blot: RAMP1 Antibody [NBP1-20966] - (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RAMP1 Antibody Summary

Immunogen
Peptide with sequence C-DYQSKRTEGIV corresponding to C-Terminus according to NP_848488.2.
Epitope
C terminus
Specificity
This antibody is expected to recognize isoform 1 (NP_058590.1) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RAMP1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Experiments gave bands at approx. 18 kDa band and 40 kDa band in rat brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in scientific literature with different antibodies (PMID: 11535606). This protein has a calculated MW of 16.9 kDa band according to mouse NP_058590.1 and 16.8 kDa band according to rat NP_113833.1.
Publications
Read Publications using
NBP1-20966 in the following applications:

  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RAMP1 Antibody

  • calcitonin receptor-like receptor activity modifying protein 1
  • Calcitonin-receptor-like receptor activity-modifying protein 1
  • CRLR activity-modifying protein 1
  • RAMP1
  • receptor (calcitonin) activity modifying protein 1
  • receptor (G protein-coupled) activity modifying protein 1
  • receptor activity modifying protein 1
  • receptor activity-modifying protein 1

Background

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RAMP1 Antibody (NBP1-20966)(4)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RAMP1
Entrez
Uniprot