Human PTH1R/PTHR1 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human PTH1R/PTHR1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39265] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.124 ng/mL (example only; lot dependent)

Human PTH1R/PTHR1 - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 0.312 - 20 ng/mL (example only; lot dependent)
• Sensitivity: 0.124 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PTH1R

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PTH1R/PTHR1 - Ready-To-Use ELISA Kit (Colorimetric)

• MGC138426
• parathyroid hormone 1 receptorMGC138452
• parathyroid hormone/parathyroid hormone-related peptide receptor
• parathyroid hormone/parathyroid hormone-related protein receptor
• PFE
• PTH/PTHr receptor
• PTH/PTHrP type I receptor
• PTH1 receptor
• PTH1R
• PTHR
• PTHR1
• PTHRPTHR1parathyroid hormone receptor 1
• seven transmembrane helix receptor

Background

PTHR1, a Parathyroid Hormone Receptor, mediates PTH-dependent regulation of mineral-ion homeostasis. It also mediates the paracrine action of PTHRP in endochondral bone formation. Mutations in this receptor are responsible for disorders of calcium and bone metabolism such as chondrodysplasia, hypercalcemia, and chronic renal failure. Three promoters, P1, P2, and P3, regulate the expression of PTHR. PTHR1 has been reported to be expressed in bone, breast, brain, colon, GI, kidney, liver, placenta, skin, umbilical cord, and uterus. ESTs have been isolated from B-cell/lung/testis, brain, breast, fetus, heart, heart/melanocyte/uterus, kidney, liver/spleen, lung, placenta, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PTH1R/PTHR1 ELISA Kit (NBP3-39265). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: PTH1R