Recombinant Human PRKD3/nPKC nu Protein

Product Discontinued

Recombinant Human PRKD3/nPKC nu Protein Summary

• Description: Recombinant protein containing 890 amino acids for Human PRKD3/nPKC nu
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(NM_005813)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60874)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PRKD3
• Purity: >80% SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 177 nmol phosphate incorporated into CREBTIDE substrate peptide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 142 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >80% SDS-PAGE

Alternate Names for Recombinant Human PRKD3/nPKC nu Protein

• EC 2.7.11
• EC 2.7.11.13
• EPK2
• EPK2PKD3
• nPKC nu
• nPKC-nu
• PKC nu
• PKC-NU
• PRKCN
• PRKCNnPKC-NU
• PRKD3
• Protein kinase C nu type
• protein kinase C, nu
• protein kinase D3
• Protein kinase EPK2
• protein-serine/threonine kinase
• serine/threonine-protein kinase D3

Background

PKCnu, also known as PKD3, is a members of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCnu is composed of 890 amino acid residues and the protein has 77.3% similarity to human PKC mu (PKCmu) and 77. 4% similarity to mouse PKD (the mouse homolog of PKCmu) (1). The PKCnu mRNA is ubiquitously expressed in various tissues and the gene is located between markers WI-9798 and D2S177 on chromosome 2p21 region.PKCnu has two putative diacylglycerol binding C1 domains, suggesting that it may participate in a novel diacylglycerol-mediated signaling pathway (2). PKCnu is trans-located to the plasma membrane and activated by the diacylglycerol mimic phorbol 12-myristate 13-acetate. PKCnu is an important physiologic target of the B-cell receptor (BCR) and exhibits robust activation after BCR engagement (2). GPCR agonists induced a rapid activation of PKCnu by a protein kinase C (PKC)-dependent pathway that leads to the phosphorylation of the activation loop of PKCnu. PKCnu is present both in the nucleus and cytoplasm and this distribution of PKCnu results from its continuous shuttling between both compartments by a mechanism that requires a nuclear import receptor and a competent CRM1-nuclear export pathway (3). Cell stimulation with the GPCR agonist neurotensin induced a rapid and reversible plasma membrane translocation of PKCnu that is PKC-dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PRKD3