POU4F3 Antibody

Western Blot: POU4F3 Antibody [NBP1-52036] - Analysis of POU4F3 in Human Heart lysate (35ug protein in RIPA buffer) at 0.2ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

POU4F3 Antibody Summary

• Immunogen: Peptide with sequence C-EAAYREKNSKPE corresponding to internal region according to NP_002691.1.
• Epitope: C-EAAYREKNSKPE
• Specificity: This antibody is expected not to cross-react to the similar POU4F1 and POU4F2.
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POU4F3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.2 - 0.6 ug/ml
• Application Notes: WB: Approx. 40 kDa band observed in human and mouse heart lysates (calculated MW of 37.1 kDa band according to human NP_002691.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POU4F3 Antibody

• brain-3C
• Brain-specific homeobox/POU domain protein 3C
• BRN3.1
• Brn-3C
• BRN3Cbrn-3C
• DFNA15
• MGC138412
• POU class 4 homeobox 3
• POU domain class 4, transcription factor 3
• POU domain, class 4, transcription factor 3

Background

This gene encodes a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. This protein is found in the retina and may play a role in determining or maintaining the identities of a small subset of visual system neurons. Defects in this gene are the cause of non-syndromic sensorineural deafness autosomal dominant type 15. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POU4F3 Antibody (NBP1-52036)(1)

Publication using NBP1-52036

• van Drunen FJ, Pauw RJ, Collin RW et al. Vestibular impairment in a Dutch DFNA15 family with an L289F mutation in POU4F3. Audiol Neurootol 2009-01-01 [PMID: 19372648]

Bioinformatics

• Gene Symbol: POU4F3
• Entrez:
  • 5459(Human)
• Uniprot:
  • NP_002691.1(Human)