POFUT2 Overexpression Lysate (Native)

Images

 
Western Blot: POFUT2 Overexpression Lysate (Adult Normal) [NBL1-14565] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for POFUT2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

POFUT2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209505 and based on accession number NM_133635. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein O-fucosyltransferase 2 (POFUT2), transcript variant 3, mRNA.
Gene
POFUT2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for POFUT2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for POFUT2 Overexpression Lysate (Native)

  • C21orf80
  • chromosome 21 open reading frame 80
  • FUT13EC 2.4.1.221
  • GDP-fucose protein O-fucosyltransferase 2
  • KIAA0958
  • O-FucT-2
  • Peptide-O-fucosyltransferase 2
  • protein O-fucosyltransferase 2

Background

Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM]. Transcript Variant: This variant (3) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (C) is longer and differs in the C-terminus compared to isoform A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol POFUT2