Pin1 Antibody (3G8)

Images

 
Western Blot: Pin1 Antibody (3G8) [NBP1-22956] Cell lysates of 293T, HeLa and Jurkat (each 50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Pin1 (1:500). Proteins were visualized ...read more
Flow Cytometry: Pin1 Antibody (3G8) [NBP1-22956] - Analysis of Pin1 in Jurkat cell line, staining at2-5ug for 1x106cells (red line). The secondary antibody usedgoat anti-mouse IgG Alexa fluor 488 conjugate. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, PAGE
Clone
3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Pin1 Antibody (3G8) Summary

Immunogen
Recombinant human Pin1 (1-163 aa) purified from E. coli
Specificity
Anti-human Pin1 mAb, clone 3G8, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Pin1 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIN1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • SDS-Page 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Pin1 Antibody (3G8)

  • dod
  • EC 5.2.1.8
  • peptidylprolyl cis/trans isomerase, NIMA-interacting 1
  • peptidyl-prolyl cis/trans isomerase, NIMA-interacting
  • peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
  • Peptidyl-prolyl cis-trans isomerase Pin1
  • Pin1
  • PPIase Pin1
  • prolyl isomerase
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
  • protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1
  • Rotamase Pin1
  • UBL5

Background

Human Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-57062
Species: Hu
Applications: WB, ICC/IF
NB110-68281
Species: Hu, Mu, Rt, Mk
Applications: ICC/IF (-), WB, Simple Western, ELISA, Flow
NBP2-38604
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
MAB3777
Species: Hu, Mu, Rt
Applications: WB
NBP2-25162
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
H00002288-M01
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P, IP
NBP2-14016
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF4094
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
NBP1-85360
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88766
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38905
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-83889
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87929
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF4174
Species: Hu, Mu, Rt
Applications: WB, Simple Western
NBP1-85921
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13794
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83887
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-46420
Species: Hu
Applications: WB, IHC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP

Publications for Pin1 Antibody (NBP1-22956) (0)

There are no publications for Pin1 Antibody (NBP1-22956).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Pin1 Antibody (NBP1-22956) (0)

There are no reviews for Pin1 Antibody (NBP1-22956). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Pin1 Antibody (NBP1-22956) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Pin1 Products

Bioinformatics Tool for Pin1 Antibody (NBP1-22956)

Discover related pathways, diseases and genes to Pin1 Antibody (NBP1-22956). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Pin1 Antibody (NBP1-22956)

Discover more about diseases related to Pin1 Antibody (NBP1-22956).
 

Pathways for Pin1 Antibody (NBP1-22956)

View related products by pathway.

PTMs for Pin1 Antibody (NBP1-22956)

Learn more about PTMs related to Pin1 Antibody (NBP1-22956).
 

Research Areas for Pin1 Antibody (NBP1-22956)

Find related products by research area.

Blogs on Pin1

There are no specific blogs for Pin1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Pin1 Antibody (3G8) and receive a gift card or discount.

Bioinformatics

Gene Symbol PIN1
Entrez
Uniprot