Recombinant Human Pyruvate Dehydrogenase Kinase 1/PDK1 Protein

Product Discontinued

Recombinant Human Pyruvate Dehydrogenase Kinase 1/PDK1 Protein Summary

• Description: Recombinant protein for Human PDK-1
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(Q15118)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: PDK1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 159 nmol phosphate incorporated into PDKtide substrate peptide per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Pyruvate Dehydrogenase Kinase 1/PDK1 Protein

• EC 2.7.11
• EC 2.7.11.2
• mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
• PDH kinase 1
• PDK1
• PDK-1
• PKB kinase
• Pyruvate dehydrogenase kinase isoform 1
• pyruvate dehydrogenase kinase, isoenzyme 1
• pyruvate dehydrogenase kinase, isozyme 1
• Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial

Background

Pyruvate dehydrogenase kinase isoform 1 (PDK1) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDK1