PCBP3 Antibody - BSA Free

Western Blot: PCBP3 Antibody [NBP1-89505] - Used 30ug of protein. Antibody diluted 1:250. WB image submitted by a verified customer review.

Immunohistochemistry-Paraffin: PCBP3 Antibody [NBP1-89505] - Staining of human duodenum shows strong cytoplasmic positivity with a granular pattern in glandular cells.

Analysis in human cell line HDLM-2.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PCBP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PCBP3 Antibody - BSA Free (NBP1-89505) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TIQGQYAIPHPDQLTKLHQLAMQQTPFPPLGQTNPAFPGEKLPLHSSEEAQNLMGQSSGLDASPPASTHELTIPNDLIGCIIGRQGTKINEIR
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PCBP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  PCBP3 Protein (NBP1-89505PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PCBP3 Antibody - BSA Free

• Alpha-CP3
• poly (rC)-binding protein 310ALPHA-CP3
• poly(rC) binding protein 3
• poly(rC)-binding protein 3

Background

PCBP3 encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for PCBP3 Antibody (NBP1-89505) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 11/18/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: fibroblasts
• Species: Human
• Lot: R29091

Comments

• Comments: 1X PBS/5% normal serum/0.3% Triton™ X-100
  Diluted 1:100 in 1X PBS/1% BSA/0.3% Triton™ X-100, 16 hours at 4C incubation.

reviewed by: Verified Customer WB Human 08/18/2017

Summary

• Application: Western Blot
• Sample Tested: Fibroblast whole cell lysate
• Species: Human
• Lot: R29091

Comments

• Comments: 30ug of protein. 4-12% Bis-Tris Gel. Incubated o/n 4C in primary 1:250 dilution.

Bioinformatics

• Gene Symbol: PCBP3