PANK4 [p Thr63] Antibody - Azide Free

Western Blot: PANK4 [p Thr63] Antibody [NBP2-60782] - PanK4 [p Thr63] Antibody [NBP2-60782] - Western blot of mouse whole brain lysate showing specific labeling of the ~86 kDa PanK4 phosphorylated at Ser63 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated with lambda phosphatase (Lambda-Ptase, 1200 units for 30 minutes).

• Reactivity: Mu, Rt, Ha
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

PANK4 [p Thr63] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit PANK4 [p Thr63] Antibody - Azide Free (NBP2-60782) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr63 conjugated to KLH. Accession # Q80YV4
• Modification: p Thr63
• Specificity: Specific for the ~86 kDa PanK4 phosphorylated at Ser63. Immunolabeling of PanK4 is nearly eliminated with lambda-phosphatase treatment.
• Predicted Species: Rat (100%), Hamster (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PANK4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for PANK4 [p Thr63] Antibody - Azide Free

• DKFZp547M242
• EC 2.7.1.33
• FLJ10782
• hPanK4
• pantothenate kinase 4
• Pantothenic acid kinase 4

Background

PANK4 encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is most abundant in muscle but is expressed in all tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PANK4