PABPC5 Antibody

Images

 
Western Blot: PABPC5 Antibody [NBP2-19672] -. Rat tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: PABPC5 Antibody [NBP2-19672] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
Western Blot: PABPC5 Antibody [NBP2-19672] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: PABPC5 Antibody [NBP2-19672] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.
Western Blot: PABPC5 Antibody [NBP2-19672] - Mouse tissue extracts (50 ug) was separated by 10 % SDS-PAGE, and the membrane was blotted with PABP5 antibody diluted by 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PABPC5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PABP5. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Bovine (95%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PABPC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-19672 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (100%), Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for PABPC5 Antibody

  • cytoplasmic 5
  • FLJ51677
  • PABP-5
  • poly(A) binding protein, cytoplasmic 5
  • Poly(A)-binding protein 5

Background

This gene encodes a protein that binds to the polyA tail found at the 3' end of most eukaryotic mRNAs. It is thought to play a role in the regulation of mRNA metabolic processes in the cytoplasm. This gene is located in a gene-poor region within the X-specific 13d-sY43 subinterval of the chromosome Xq21.3/Yp11.2 homology block. It is located close to translocation breakpoints associated with premature ovarian failure, and is therefore a potential candidate gene for this disorder. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Review for PABPC5 Antibody (NBP2-19672) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-19672:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot PABPC5 NBP2-19672
Enlarge
3
reviewed by:
Anonymous
WB Human 05/12/2016
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Summary

ApplicationWestern Blot
Sample Testedhuman lymphocytes, whole cell lysate
SpeciesHuman
Lot40380

Blocking

Blocking Details5% Milk/TBS-T, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:1000 in 5% Milk, TBS-T, 16H, 4C

Secondary Antibody

Secondary DescriptionAmersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey)
Secondary Manufacturer Cat#NA934-100UL
Secondary Concentration1:5000

Details

Detection NotesECL, long exposure (1 hour)

Comments

CommentsStrong bands at 65 and 55kDA, specific 43kDa band visible after a long exposure.

Product General Protocols

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Video Protocols

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FAQs for PABPC5 Antibody (NBP2-19672) (0)

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Secondary Antibodies

 

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Recent Reviews

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5
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1
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1
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Anonymous
05/12/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol PABPC5
Uniprot